ICP-MS-Based Multiplex and Ultrasensitive Assay of Viruses with Lanthanide-Coded Biospecific Tagging and Amplification Strategies

CORC > 厦门大学 > 化学化工－已发表论文

	ICP-MS-Based Multiplex and Ultrasensitive Assay of Viruses with Lanthanide-Coded Biospecific Tagging and Amplification Strategies
	Luo, Yacui ; Yan, Xiaowen ; Huang, Yishun ; Wen, Ruibin ; Li, Zhaoxin ; Yang, Limin ; Yang, Chaoyong James ; Wang, Qiuquan ; Yang CY(杨朝勇) ; Wang QQ(王秋泉)
刊名	http://dx.doi.org/10.1021/ac402446a
	2013
关键词	PLASMA-MASS SPECTROMETRY ROLLING-CIRCLE AMPLIFICATION ABSOLUTE QUANTIFICATION NUCLEIC-ACIDS SENSITIVE DETECTION DNA DETECTION QUANTUM DOTS PATHOGEN DNA NANOPARTICLES PROTEINS
英文摘要	National Natural Science Foundation of China [21035006, 21275120]; National Basic Research 973 Project [2014CB932004]; Highly sensitive and a multiplex assay of viruses and viral DNAs in complex biological samples is extremely important for clinical diagnosis and prognosis of pathogenic diseases as well as virology studies. We present an effective ICP-MS-based multiplex and ultrasensitive assay of viral DNAs with lanthanide-coded oligonucleotide hybridization and rolling circle amplification (RCA) strategies on biofunctional magnetic nanoparticles (MNPs), in which single-stranded capture DNA (ss-Cap-DNA)-functionalized MNPs (up to 1.65 X 10(4) ss-Cap-DNA per MNP) were used to recognize and enrich target DNAs, and single-stranded report DNA (ss-Rep-DNA-DOTA-Ln) coded by the lanthanide-DOTA complex hybridized with the targeted DNA for highly sensitive readout of HIV (28 amol), HAV (48 amol), and HBV (19 amol). When utilizing the RCA technique in association with the design and synthesis of a "bridge" DNA and a corresponding ss-Rep-DNA-DOTA-Ho, as low as 90 zmol HBV could be detected. Preliminary applications to the determination of the viral DNAs in 4T1 cell lysates and in serum confirmed the feasibility of this ICP-MS-based multiplex DNA assay for clinical use. One can expect that this element-coded ICP-MS-based multiplex and ultrasensitive DNA assay will play an ever more important role in the fields of bioanalysis and virology and in medical studies after further sophisticated modifications.
语种	英语
出版者	AMER CHEMICAL SOC
内容类型	期刊论文
源URL	[http://dspace.xmu.edu.cn/handle/2288/88856]
专题	化学化工－已发表论文
推荐引用方式 GB/T 7714	Luo, Yacui,Yan, Xiaowen,Huang, Yishun,et al. ICP-MS-Based Multiplex and Ultrasensitive Assay of Viruses with Lanthanide-Coded Biospecific Tagging and Amplification Strategies[J]. http://dx.doi.org/10.1021/ac402446a,2013.
APA	Luo, Yacui.,Yan, Xiaowen.,Huang, Yishun.,Wen, Ruibin.,Li, Zhaoxin.,...&王秋泉.(2013).ICP-MS-Based Multiplex and Ultrasensitive Assay of Viruses with Lanthanide-Coded Biospecific Tagging and Amplification Strategies.http://dx.doi.org/10.1021/ac402446a.
MLA	Luo, Yacui,et al."ICP-MS-Based Multiplex and Ultrasensitive Assay of Viruses with Lanthanide-Coded Biospecific Tagging and Amplification Strategies".http://dx.doi.org/10.1021/ac402446a (2013).