Genes encoding the production of extracellular polysaccharide bioflocculant are clustered on a 30-kb DNA segment in Bacillus licheniformis

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	Genes encoding the production of extracellular polysaccharide bioflocculant are clustered on a 30-kb DNA segment in Bacillus licheniformis
	Yan, Shan ; Wang, Na ; Chen, Zhen ; Wang, Yuanpeng ; He, Ning ; He N(何宁) ; Peng, Yajuan ; Li, Qingbiao ; Li QB(李清彪) ; Deng, Xu
刊名	http://dx.doi.org/10.1007/s10142-013-0333-4
	2013
关键词	STREPTOCOCCUS-MUTANS BACTERIAL EXOPOLYSACCHARIDES BIOPOLYMER FLOCCULANT BIOFILM FORMATION SUBSTANCES EPS BIOSYNTHESIS GALACTOSE SUBTILIS IDENTIFICATION GENOME
英文摘要	National Basic Research Program of China [2013CB733505]; Xiamen Science and Technology committee [3502Z20121021]; National natural science foundation of China [51378444]; Shenzhen Science and Technology Research Project [JC201005280412A]; Bioflocculants are special high-molecular weight polymers produced by microorganisms. Despite the fact that several types of bioflocculants from different species of bacteria have been reported, there is a large gap in our knowledge regarding the molecular machine responsible for the production of bioflocculants. To investigate genes involved in bioflocculant synthesis, a fosmid library was generated from Bacillus licheniformis genomic DNA and screened for the production of bioflocculant. Four positive clones with distinct flocculation were isolated by a two-pooling scheme. The clone with 662 U ml(-1) flocculating activity was sequenced. As a result, a 30-kb fragment with 26 hypothetical genes was identified in the bioflocculant-producing clone. Most of the predicted proteins encoded by the inserted genes showed significant homology with enzymes involved in the biosynthesis of polysaccharide. Based on these homologies, a biosynthesis pathway and two gene clusters involved in the production of the polysaccharide bioflocculant were proposed with the integration of functional descriptions of individual genes by metabolic databases, and a glucose-sensitive glycosidases was predicted. This research supplied significant data for potential application of bioflocculant-producing strains in wastewater refining and industrial downstream treatments.
语种	英语
出版者	SPRINGER HEIDELBERG
内容类型	期刊论文
源URL	[http://dspace.xmu.edu.cn/handle/2288/88842]
专题	化学化工－已发表论文
推荐引用方式 GB/T 7714	Yan, Shan,Wang, Na,Chen, Zhen,et al. Genes encoding the production of extracellular polysaccharide bioflocculant are clustered on a 30-kb DNA segment in Bacillus licheniformis[J]. http://dx.doi.org/10.1007/s10142-013-0333-4,2013.
APA	Yan, Shan.,Wang, Na.,Chen, Zhen.,Wang, Yuanpeng.,He, Ning.,...&Deng, Xu.(2013).Genes encoding the production of extracellular polysaccharide bioflocculant are clustered on a 30-kb DNA segment in Bacillus licheniformis.http://dx.doi.org/10.1007/s10142-013-0333-4.
MLA	Yan, Shan,et al."Genes encoding the production of extracellular polysaccharide bioflocculant are clustered on a 30-kb DNA segment in Bacillus licheniformis".http://dx.doi.org/10.1007/s10142-013-0333-4 (2013).