Distinct Metabolic Profile of Primary Focal Segmental Glomerulosclerosis Revealed by NMR-Based Metabolomics

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	Distinct Metabolic Profile of Primary Focal Segmental Glomerulosclerosis Revealed by NMR-Based Metabolomics
	Hao, Xu ; Liu, Xia ; Wang, Weiming ; Ren, Hong ; Xie, Jingyuan ; Shen, Pingyan ; Lin, Donghai ; Chen, Nan ; Lin DH(林东海)
刊名	http://dx.doi.org/10.1371/journal.pone.0078531
	2013
关键词	RENAL ORGANIC ANION GLYCINE CONJUGATION BENZOIC-ACID OXIDATIVE STRESS UREMIC TOXINS RAT-KIDNEY METABONOMICS 3-METHYLHISTIDINE TRANSPORT URINE
英文摘要	National Basic Research Program of China 973 Program [2012CB517600, 2012CB517604]; National Natural Science Foundation of China [81070568, 81000295, 81370015]; Shanghai Pujiang Program [11PJ1406700]; Background: Primary focal segmental glomerulosclerosis (FSGS) is pathological entity which is characterized by idiopathic steroid-resistant nephrotic syndrome (SRNS) and progression to end-stage renal disease (ESRD) in the majority of affected individuals. Currently, there is no practical noninvasive technique to predict different pathological types of glomerulopathies. In this study, the role of urinary metabolomics in the diagnosis and pathogenesis of FSGS was investigated. Methods: NMR-based metabolomics was applied for the urinary metabolic profile in the patients with FSGS (n = 25), membranous nephropathy (MN, n = 24), minimal change disease (MCD, n = 14) and IgA nephropathy (IgAN, n = 26), and healthy controls (CON, n = 35). The acquired data were analyzed using principal component analysis (PCA) followed by orthogonal projections to latent structure discriminant analysis (OPLS-DA). Model validity was verified using permutation tests. Results: FSGS patients were clearly distinguished from healthy controls and other three types of glomerulopathies with good sensitivity and specificity based on their global urinary metabolic profiles. In FSGS patients, urinary levels of glucose, dimethylamine and trimethylamine increased compared with healthy controls, while pyruvate, valine, hippurate, isoleucine, phenylacetylglycine, citrate, tyrosine, 3-methylhistidine and b-hydroxyisovalerate decreased. Additionally, FSGS patients had lower urine N-methylnicotinamide levels compared with other glomerulopathies. Conclusions: NMR-based metabonomic approach is amenable for the noninvasive diagnosis and differential diagnosis of FSGS as well as other glomerulopathies, and it could indicate the possible mechanisms of primary FSGS.
语种	英语
出版者	PUBLIC LIBRARY SCIENCE
内容类型	期刊论文
源URL	[http://dspace.xmu.edu.cn/handle/2288/88824]
专题	化学化工－已发表论文
推荐引用方式 GB/T 7714	Hao, Xu,Liu, Xia,Wang, Weiming,et al. Distinct Metabolic Profile of Primary Focal Segmental Glomerulosclerosis Revealed by NMR-Based Metabolomics[J]. http://dx.doi.org/10.1371/journal.pone.0078531,2013.
APA	Hao, Xu.,Liu, Xia.,Wang, Weiming.,Ren, Hong.,Xie, Jingyuan.,...&林东海.(2013).Distinct Metabolic Profile of Primary Focal Segmental Glomerulosclerosis Revealed by NMR-Based Metabolomics.http://dx.doi.org/10.1371/journal.pone.0078531.
MLA	Hao, Xu,et al."Distinct Metabolic Profile of Primary Focal Segmental Glomerulosclerosis Revealed by NMR-Based Metabolomics".http://dx.doi.org/10.1371/journal.pone.0078531 (2013).