An etched porous interface for on-line capillary electrophoresis-based two-dimensional separation system

	An etched porous interface for on-line capillary electrophoresis-based two-dimensional separation system
	Liu, HC; Zhang, LH; Zhu, GJ; Zhang, WB; Zhang, YK
刊名	analytical chemistry
	2004-11-01
卷号	76 期号:21 页码:6506-6512
产权排序	1;1
英文摘要	the construction and evaluation of an on-column etched fused-silica porous junction for on-line coupling of capillary isoelectric focusing (cief) with capillary zone electrophoresis (cze) are described. where two separation columns were integrated on a single piece of fused-silica capillary through the etched similar to4 to 5-mm length porous junction along the capillary. the junction is easily prepared by etching a short section of the capillary wall with hf after removing the polyimide coating. the etched section becomes a porous glass membrane that allows only small ions related to the background electrolyte to pass through when high voltage is applied across the separation capillary. the primary advantages of this novel porous junction interface over previous designs (in which the interface is usually formed by fracturing the capillary followed by connecting the two capillaries with a section of microdialysis hollow fiber membrane) are no dead volume, simplicity, and ruggedness, which is particularly well suited for an on-line coupling capillary electrophoresis-based multiple dimensional separation system. the performance of the 2d cief-cze system constructed by such an etched porous junction was evaluated by the analyses of protein mixtures.
WOS标题词	science & technology ; physical sciences
类目[WOS]	chemistry, analytical
研究领域[WOS]	chemistry
关键词[WOS]	flow gating interface ; zone-electrophoresis ; mass-spectrometry ; gel-electrophoresis ; microcolumn-lc ; chromatography ; peptides ; cze
收录类别	SCI
原文出处	true
语种	英语
WOS记录号	WOS:000224841300051
公开日期	2010-11-30
内容类型	期刊论文
源URL	[http://159.226.238.44/handle/321008/80973]
专题	大连化学物理研究所_中国科学院大连化学物理研究所
作者单位	Chinese Acad Sci, Dalian Inst Chem Phys, Natl Chromatog R&A Ctr, Dalian 116011, Peoples R China
推荐引用方式 GB/T 7714	Liu, HC,Zhang, LH,Zhu, GJ,et al. An etched porous interface for on-line capillary electrophoresis-based two-dimensional separation system[J]. analytical chemistry,2004,76(21):6506-6512.
APA	Liu, HC,Zhang, LH,Zhu, GJ,Zhang, WB,&Zhang, YK.(2004).An etched porous interface for on-line capillary electrophoresis-based two-dimensional separation system.analytical chemistry,76(21),6506-6512.
MLA	Liu, HC,et al."An etched porous interface for on-line capillary electrophoresis-based two-dimensional separation system".analytical chemistry 76.21(2004):6506-6512.