Development of an amperometric glucose biosensor based on the immobilization of glucose oxidase in an ormosil-PVA matrix onto a Prussian Blue modified electrode

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	Development of an amperometric glucose biosensor based on the immobilization of glucose oxidase in an ormosil-PVA matrix onto a Prussian Blue modified electrode
	Chen, H. L. ; Zhao, L. ; Chen, X. ; Zhuang, Z. X. ; Wang, X. R. ; Chen X(陈曦)
刊名	http://dx.doi.org/10.1007/s11426-009-0146-4
	2009-08
关键词	QUARTZ-CRYSTAL MICROBALANCE COVALENT IMMOBILIZATION PLATINUM-ELECTRODE FILM SENSOR MEMBRANE BOD FERRICYANIDE MOLECULES SURFACE
英文摘要	National High Technical Development Project (863 project) Foundation [2006AA09Z160]; National Natural Science Foundation of China [20775064]; An amperometric glucose biosensor was developed based on the immobilization of glucose oxidase in the organically modified silicate (ormosil)-polyvinyl acetate (PVA) matrix onto a Prussian Blue (PB)-modified glassy carbon electrode. A higher stability PB-modified electrode was prepared by the electrochemical deposition of FeCl(3), K(3)[Fe(CN)(6)] and ethylenediamine tetraacetic acid (EDTA) under cyclic voltammetric (CV) conditions. The effects of the potential range of CV conditions, electrolyte cations, applied potential, pH, temperature and co-existing substances were investigated. The detection limit of the glucose biosensor was 8.1 mu mol center dot L(-1) (S/N = 3) with a linear range from 20 mu mol center dot L(-1) to 2 mmol center dot L(-1) (R = 0.9965). The biosensor presented a fast response and good selectivity. Additionally, excellent reproducibility and stability of the biosensor were observed.
语种	英语
内容类型	期刊论文
源URL	[http://dspace.xmu.edu.cn/handle/2288/64153]
专题	化学化工－已发表论文
推荐引用方式 GB/T 7714	Chen, H. L.,Zhao, L.,Chen, X.,et al. Development of an amperometric glucose biosensor based on the immobilization of glucose oxidase in an ormosil-PVA matrix onto a Prussian Blue modified electrode[J]. http://dx.doi.org/10.1007/s11426-009-0146-4,2009.
APA	Chen, H. L.,Zhao, L.,Chen, X.,Zhuang, Z. X.,Wang, X. R.,&陈曦.(2009).Development of an amperometric glucose biosensor based on the immobilization of glucose oxidase in an ormosil-PVA matrix onto a Prussian Blue modified electrode.http://dx.doi.org/10.1007/s11426-009-0146-4.
MLA	Chen, H. L.,et al."Development of an amperometric glucose biosensor based on the immobilization of glucose oxidase in an ormosil-PVA matrix onto a Prussian Blue modified electrode".http://dx.doi.org/10.1007/s11426-009-0146-4 (2009).