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	Probing free radical damage to biological molecules by spin-labeling fluorophore
	Dang, Y. M. ; Guo, X. Q. ; Zhao, Y. B. ; Guo XQ(郭祥群)
	2004-07
关键词	EXCITED SINGLET-STATES HYDROXYL RADICALS NITROXIDE DNA SUPEROXIDE
英文摘要	A spin-labeling fluorophore(NA-TEMPO.), formed by labeling a-naphthalene with a paramagnetic nitroxide (4-hydroxyl-2, 2, 6, 6-tetramethylpiperidinyl-1-oxy), was explored for biologically relevant carbon-centered radicals. The horseradish peroxidase (HPR)/H2O2 reaction was used to generate radicals in derivatives of tyrosine and phenylalanine, and the Fenton reaction was used to generate radicals in deoxyguanosine, guanosine, bovine serum albumin (BSA) and DNA. TEMPO.-NA, a weak fluorescent compound, showed a dramatic increase in fluorescence intensity after scavenging carbon-centered radicals. The spin-labeling fluorophore with dual function groups, the radical recognizing group and fluorescent group, would be significantly useful in monitoring the formation and translocation of carbon-centered radicals in complex biological systems.
语种	英语
内容类型	期刊论文
源URL	[http://dspace.xmu.edu.cn/handle/2288/64058]
专题	化学化工－已发表论文
推荐引用方式 GB/T 7714	Dang, Y. M.,Guo, X. Q.,Zhao, Y. B.,et al. Probing free radical damage to biological molecules by spin-labeling fluorophore[J],2004.
APA	Dang, Y. M.,Guo, X. Q.,Zhao, Y. B.,&郭祥群.(2004).Probing free radical damage to biological molecules by spin-labeling fluorophore..
MLA	Dang, Y. M.,et al."Probing free radical damage to biological molecules by spin-labeling fluorophore".(2004).

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