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	Preparation of temperature-sensitive polymer with regulated phase transition temperature and its application in the fluoroimmunoassay
	Zhang,CG ; Yang,HH ; Zheng,H ; Lin,P ; Xu,JG ; Li,DH ; Zheng H(郑洪)
	2003-01
关键词	FLUORESCENCE IMMUNOASSAY SEPARATING POLYMER HYDROGEL
英文摘要	A novel temperature-sensitive polymer, poly(N-isopropylacrylamide-co-acrylamide) [P(NIP-co-AA)], was synthesized.. Its lower critical solution temperature (LCST) could be regulated by changing the content of acrylamide for different purposes. The P(NIP-co-AA) with phase transition temperature (T-tr) of 37degreesC was used as a separating carrier for the reactants in immunoassay and a sandwich fluorescence immunoassay for rabbit IgG was established. The sensitivity of this method was comparable to that using poly-N-isopropylacrylamide (PNIP) as a carrier. However, owing to the elevation of T-tr of the polymer, the immunoreaction could be carried out at a temperature closer to the physiological environment of organism and the rate of inummoreaction was faster. The linear range of determination was 0 similar to 1000 mug/L and the limit of detection was 10 mug/L. This method has been applied to the determination of the rabbit IgG levels in rabbit serum with satisfactory results.
语种	英语
内容类型	期刊论文
源URL	[http://dspace.xmu.edu.cn/handle/2288/64043]
专题	化学化工－已发表论文
推荐引用方式 GB/T 7714	Zhang,CG,Yang,HH,Zheng,H,et al. Preparation of temperature-sensitive polymer with regulated phase transition temperature and its application in the fluoroimmunoassay[J],2003.
APA	Zhang,CG.,Yang,HH.,Zheng,H.,Lin,P.,Xu,JG.,...&郑洪.(2003).Preparation of temperature-sensitive polymer with regulated phase transition temperature and its application in the fluoroimmunoassay..
MLA	Zhang,CG,et al."Preparation of temperature-sensitive polymer with regulated phase transition temperature and its application in the fluoroimmunoassay".(2003).

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