L-DNA Molecular Beacon: A Safe, Stable, and Accurate Intracellular Nano-thermometer for Temperature Sensing in Living Cells | |
Ke, Guoliang ; Wang, Chunming ; Ge, Yun ; Zheng, Nanfeng ; Zhu, Zhi ; Yang, Chaoyong James ; Zheng NF(郑南峰) | |
刊名 | http://dx.doi.org/10.1021/ja3082439
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2012-11-21 | |
关键词 | NUCLEIC-ACIDS FLUORESCENT PROTEIN BIOLOGICAL SAMPLES NANOTHERMOMETERS THERMOGENESIS NANOCRYSTALS NANOSCALE DESIGN CANCER PROBES |
英文摘要 | National Basic Research Program of China [2010CB732402]; National Science Foundation of China [21075104]; National Instrumentation Program [2011YQ03012412]; Natural Science Foundation of Fujian Province for Distinguished Young Scholars [2010J06004]; National Fund for Fostering Talents of Basic Science [J1030415]; Noninvasive and accurate measurement of intracellular temperature is of great significance in biology and medicine. This paper describes a safe, stable, and accurate intracellular nano-thermometer based on an L-DNA molecular beacon (L-MB), a dual-labeled hairpin oligonucleotide built from the optical isomer of naturally occurring D-DNA. Relying on the temperature responsive hairpin structure and the FRET signaling mechanism of MBs, the fluorescence of L-MBs is quenched below the melting temperature and enhanced with increasing temperature. Because of the excellent reversibility and tunable response range, L-MBs are very suitable for temperature sensing. More importantly, the non-natural L-DNA backbone prevents the L-MBs from binding to cellular nucleic acids and proteins as well as from being digested by nucleases inside the cells, thus ensuring excellent stability and accuracy of the nano-thermometer in a complex cellular environment. The L-MB nano-thermometer was used for the photothermal study of Pd nanosheets in living cells, establishing the nano-thermometer as a useful tool for intracellular temperature measurement. |
语种 | 英语 |
内容类型 | 期刊论文 |
源URL | [http://dspace.xmu.edu.cn/handle/2288/64009] ![]() |
专题 | 化学化工-已发表论文 |
推荐引用方式 GB/T 7714 | Ke, Guoliang,Wang, Chunming,Ge, Yun,et al. L-DNA Molecular Beacon: A Safe, Stable, and Accurate Intracellular Nano-thermometer for Temperature Sensing in Living Cells[J]. http://dx.doi.org/10.1021/ja3082439,2012. |
APA | Ke, Guoliang.,Wang, Chunming.,Ge, Yun.,Zheng, Nanfeng.,Zhu, Zhi.,...&郑南峰.(2012).L-DNA Molecular Beacon: A Safe, Stable, and Accurate Intracellular Nano-thermometer for Temperature Sensing in Living Cells.http://dx.doi.org/10.1021/ja3082439. |
MLA | Ke, Guoliang,et al."L-DNA Molecular Beacon: A Safe, Stable, and Accurate Intracellular Nano-thermometer for Temperature Sensing in Living Cells".http://dx.doi.org/10.1021/ja3082439 (2012). |
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