L-DNA Molecular Beacon: A Safe, Stable, and Accurate Intracellular Nano-thermometer for Temperature Sensing in Living Cells

CORC > 厦门大学 > 化学化工－已发表论文

	L-DNA Molecular Beacon: A Safe, Stable, and Accurate Intracellular Nano-thermometer for Temperature Sensing in Living Cells
	Ke, Guoliang ; Wang, Chunming ; Ge, Yun ; Zheng, Nanfeng ; Zhu, Zhi ; Yang, Chaoyong James ; Zheng NF(郑南峰)
刊名	http://dx.doi.org/10.1021/ja3082439
	2012-11-21
关键词	NUCLEIC-ACIDS FLUORESCENT PROTEIN BIOLOGICAL SAMPLES NANOTHERMOMETERS THERMOGENESIS NANOCRYSTALS NANOSCALE DESIGN CANCER PROBES
英文摘要	National Basic Research Program of China [2010CB732402]; National Science Foundation of China [21075104]; National Instrumentation Program [2011YQ03012412]; Natural Science Foundation of Fujian Province for Distinguished Young Scholars [2010J06004]; National Fund for Fostering Talents of Basic Science [J1030415]; Noninvasive and accurate measurement of intracellular temperature is of great significance in biology and medicine. This paper describes a safe, stable, and accurate intracellular nano-thermometer based on an L-DNA molecular beacon (L-MB), a dual-labeled hairpin oligonucleotide built from the optical isomer of naturally occurring D-DNA. Relying on the temperature responsive hairpin structure and the FRET signaling mechanism of MBs, the fluorescence of L-MBs is quenched below the melting temperature and enhanced with increasing temperature. Because of the excellent reversibility and tunable response range, L-MBs are very suitable for temperature sensing. More importantly, the non-natural L-DNA backbone prevents the L-MBs from binding to cellular nucleic acids and proteins as well as from being digested by nucleases inside the cells, thus ensuring excellent stability and accuracy of the nano-thermometer in a complex cellular environment. The L-MB nano-thermometer was used for the photothermal study of Pd nanosheets in living cells, establishing the nano-thermometer as a useful tool for intracellular temperature measurement.
语种	英语
内容类型	期刊论文
源URL	[http://dspace.xmu.edu.cn/handle/2288/64009]
专题	化学化工－已发表论文
推荐引用方式 GB/T 7714	Ke, Guoliang,Wang, Chunming,Ge, Yun,et al. L-DNA Molecular Beacon: A Safe, Stable, and Accurate Intracellular Nano-thermometer for Temperature Sensing in Living Cells[J]. http://dx.doi.org/10.1021/ja3082439,2012.
APA	Ke, Guoliang.,Wang, Chunming.,Ge, Yun.,Zheng, Nanfeng.,Zhu, Zhi.,...&郑南峰.(2012).L-DNA Molecular Beacon: A Safe, Stable, and Accurate Intracellular Nano-thermometer for Temperature Sensing in Living Cells.http://dx.doi.org/10.1021/ja3082439.
MLA	Ke, Guoliang,et al."L-DNA Molecular Beacon: A Safe, Stable, and Accurate Intracellular Nano-thermometer for Temperature Sensing in Living Cells".http://dx.doi.org/10.1021/ja3082439 (2012).