Antisense therapy targeting pokemon oncogene in MCF-7 cells

CORC > 厦门大学 > 化学化工－已发表论文

	Antisense therapy targeting pokemon oncogene in MCF-7 cells
	Zhang, N. ; Xie, Z. H. ; Jiang, Y. Y. ; Zhao, Y. F. ; Zhao YF(赵玉芬)
刊名	http://dx.doi.org/10.1080/10426500701807822
	2008
关键词	PLZF
英文摘要	This study is undertaken to investigate the role of Pokemon oncogene in MCF-7 cells growth and the potential of Pokemon as a target for cancer therapy. An 18-mer antisense oligonucleotide As4 (YTCAGGTCGTAGTTGTGG, targeting 1496-1513 site of Pokemon mRNA) has been proven as a potent inhibitor of Pokemon expression. in our previous work. In this study, we transfected As4 to MCF-7cells and confirmed the Pokemon mRNA and protein level by RT-PCR and western blot. Furthermore, the cell cycle has been analyzed by flow cytometer. G2/M arrest has been observed when, the cells were treated with antisense oligonucleotide at 100 nM for 48 h. We've also found the P53 protein upregulation due to Pokemon inhibition. These results suggest that Pokemon inhibitor may be a novel approach to human cancer therapy.
语种	英语
内容类型	期刊论文
源URL	[http://dspace.xmu.edu.cn/handle/2288/63732]
专题	化学化工－已发表论文
推荐引用方式 GB/T 7714	Zhang, N.,Xie, Z. H.,Jiang, Y. Y.,et al. Antisense therapy targeting pokemon oncogene in MCF-7 cells[J]. http://dx.doi.org/10.1080/10426500701807822,2008.
APA	Zhang, N.,Xie, Z. H.,Jiang, Y. Y.,Zhao, Y. F.,&赵玉芬.(2008).Antisense therapy targeting pokemon oncogene in MCF-7 cells.http://dx.doi.org/10.1080/10426500701807822.
MLA	Zhang, N.,et al."Antisense therapy targeting pokemon oncogene in MCF-7 cells".http://dx.doi.org/10.1080/10426500701807822 (2008).