DETERMINATION OF THIAMINE (VITAMIN-B1) BY INSITU SENSITIZED PHOTOCHEMICAL SPECTROFLUOROMETRY

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	DETERMINATION OF THIAMINE (VITAMIN-B1) BY INSITU SENSITIZED PHOTOCHEMICAL SPECTROFLUOROMETRY
	HUANG,XZ ; CHEN,GZ ; XU,JG ; ZHAO,YB ; WU,YZ ; GUO,XQ ; Zhao YB(赵一兵)
刊名	http://dx.doi.org/10.1016/0003-2670(93)85050-T
	1993-04-15
关键词	PERFORMANCE LIQUID-CHROMATOGRAPHY RIBOFLAVIN THIOCHROME DERIVATIZATION DERIVATIVES PYRIDOXINE ELECTRODES FOODS BLOOD
英文摘要	An in situ photochemical spectrofluorimetric flow-injection method for the determination of thiamine (vitamin B1) is proposed. It is based on the conversion of thiamine in alkaline medium into an intensively fluorescent compound. The photochemical reaction is sensitized by acetone. The determination can be carried out by measuring the fluorescence intensity when the fluorescent signal reaches its maximum or at a fixed time. The calibration graph was linear up to 10.0 mug ml-1 thiamine (r = 0.9996), the limit of detection was 0.46 ng ml-1 thiamine and the relative standard deviation was 1.5% for 50.0 ng ml-1 thiamine (n = 6). The kinetic behaviour of the reaction and the effects of some experimental conditions were investigated and are discussed in detail. The mechanism of the sensitization of acetone was examined. Its application to vitamin B1 tablets and injections was found to be satisfactory. By use of a selection valve and combination with a synchronous fluorescence technique, vitamin B1, B2 and B6 in pharmaceuticals were simultaneously determined.
语种	英语
内容类型	期刊论文
源URL	[http://dspace.xmu.edu.cn/handle/2288/63389]
专题	化学化工－已发表论文
推荐引用方式 GB/T 7714	HUANG,XZ,CHEN,GZ,XU,JG,et al. DETERMINATION OF THIAMINE (VITAMIN-B1) BY INSITU SENSITIZED PHOTOCHEMICAL SPECTROFLUOROMETRY[J]. http://dx.doi.org/10.1016/0003-2670(93)85050-T,1993.
APA	HUANG,XZ.,CHEN,GZ.,XU,JG.,ZHAO,YB.,WU,YZ.,...&赵一兵.(1993).DETERMINATION OF THIAMINE (VITAMIN-B1) BY INSITU SENSITIZED PHOTOCHEMICAL SPECTROFLUOROMETRY.http://dx.doi.org/10.1016/0003-2670(93)85050-T.
MLA	HUANG,XZ,et al."DETERMINATION OF THIAMINE (VITAMIN-B1) BY INSITU SENSITIZED PHOTOCHEMICAL SPECTROFLUOROMETRY".http://dx.doi.org/10.1016/0003-2670(93)85050-T (1993).