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	Purification and characterization of an extracellular laccase from the anthracene-degrading fungus Fusarium solani MAS2
	Luo,ZH ; Chow,RKK ; Vrijmoed,LLP ; Wu,YR ; Luo ZH(罗正鸿)
	2010
关键词	Polycyclic aromatic hydrocarbons (PAHs) ABTS Heavy metal tolerance Mangrove sediment
英文摘要	An extracellular laccase was purified from the culture medium of the non-white rot, anthracene-degrading fungal strain Fusarium solani MAS2. Both native PAGE and SDS-PAGE revealed one single band corresponding to a molecular weight of about 72 kDa. Treatment with endoglycosidase H reduced the molecular weight by 12%. The purified laccase maintained stable at pH 3-11 and up to 50 degrees C. The highest activity was detected at pH 3.0 and at 70 degrees C. The enzyme retained 46.2-97.2% of it activity in the presence of 20 mM Pb(2+), Ni(2+), Cr(3+), and its activity was enhanced in the presence of 20 mM Hg(2+). The laccase retained more than 50% of its activity in the presence of 5% acetone, acetonitrile, dimethyl sulphoxide (DMSO), ethanol and methanol. The kinetic constants (K(m) and K(cat)) showed that 2,6-dimethoxyphenol (DMOP) and 2,2'-azino-bis-(3-ethylbenzthiazoline-6-sulphonic acid) diammonium salt (ABTS) were the more effective substrates rather than catechol and guaiacol. The novel properties of this laccase suggest its potential for biotechnological and environmental applications. (C) 2010 Elsevier Ltd. All rights reserved.
语种	英语
内容类型	期刊论文
源URL	[http://dspace.xmu.edu.cn/handle/2288/63144]
专题	化学化工－已发表论文
推荐引用方式 GB/T 7714	Luo,ZH,Chow,RKK,Vrijmoed,LLP,et al. Purification and characterization of an extracellular laccase from the anthracene-degrading fungus Fusarium solani MAS2[J],2010.
APA	Luo,ZH,Chow,RKK,Vrijmoed,LLP,Wu,YR,&罗正鸿.(2010).Purification and characterization of an extracellular laccase from the anthracene-degrading fungus Fusarium solani MAS2..
MLA	Luo,ZH,et al."Purification and characterization of an extracellular laccase from the anthracene-degrading fungus Fusarium solani MAS2".(2010).

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