Europium-Labeled Activity-Based Probe through Click Chemistry: Absolute Serine Protease Quantification Using 153Eu Isotope Dilution ICP/MS

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	Europium-Labeled Activity-Based Probe through Click Chemistry: Absolute Serine Protease Quantification Using 153Eu Isotope Dilution ICP/MS
	Yan, Xiaowen ; Luo, Yacui ; Zhang, Zhubao ; Li, Zhaoxin ; Luo, Qiang ; Yang, Limin ; Zhang, Bo ; Chen, Haifeng ; Bai, Peiming ; Wang, Qiuquan ; Wang QQ(王秋泉)
刊名	http://dx.doi.org/10.1002/anie.201108277
	2012
关键词	ELEMENT MASS-SPECTROMETRY PHENYLMETHANESULFONYL ALPHA-CHYMOTRYPSIN ANION-EXCHANGE CHROMATOGRAPHY QUANTITATIVE-ANALYSIS HUMAN SERUM SULFONYL FLUORIDES NANOHPLC-ICPMS HPLC-ICPMS PROTEINS MS
英文摘要	National Natural Science Foundation of China [21035006]; National Basic Research 973 Program [2009CB421605]; The titled probe was synthesized by conjugating a sulfonyl fluoride and azido unit using click chemistry to give SF-Eu, which can react specifically with serine (Ser) in the active site of serine protease (SP). Combination of the method with 153Eu-isotope dilution ICP/MS enables absolute protein quantification of active SPs in biological samples using only one 153Eu(NO3)3 isotopic standard.
语种	英语
内容类型	期刊论文
源URL	[http://dspace.xmu.edu.cn/handle/2288/62792]
专题	化学化工－已发表论文
推荐引用方式 GB/T 7714	Yan, Xiaowen,Luo, Yacui,Zhang, Zhubao,et al. Europium-Labeled Activity-Based Probe through Click Chemistry: Absolute Serine Protease Quantification Using 153Eu Isotope Dilution ICP/MS[J]. http://dx.doi.org/10.1002/anie.201108277,2012.
APA	Yan, Xiaowen.,Luo, Yacui.,Zhang, Zhubao.,Li, Zhaoxin.,Luo, Qiang.,...&王秋泉.(2012).Europium-Labeled Activity-Based Probe through Click Chemistry: Absolute Serine Protease Quantification Using 153Eu Isotope Dilution ICP/MS.http://dx.doi.org/10.1002/anie.201108277.
MLA	Yan, Xiaowen,et al."Europium-Labeled Activity-Based Probe through Click Chemistry: Absolute Serine Protease Quantification Using 153Eu Isotope Dilution ICP/MS".http://dx.doi.org/10.1002/anie.201108277 (2012).