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	Synthesis and investigation of deoxyribonucleic acid/locked nucleic acid chimeric molecular beacons
	Yang CY(杨朝勇)
	2007
关键词	SOLID-PHASE SYNTHESIS PNA-DNA-HYBRIDS LIVING CELLS MESSENGER-RNAS LNA PROBES HYBRIDIZATION OLIGONUCLEOTIDES FLUORESCENCE RECOGNITION
英文摘要	To take full advantage of locked nucleic acid (LNA) based molecular beacons (LNA-MBs) for a variety of applications including analysis of complex samples and intracellular monitoring, we have systematically synthesized a series of DNA/LNA chimeric MBs and studied the effect of DNA/LNA ratio in MBs on their thermodynamics, hybridization kinetics, protein binding affinity and enzymatic resistance. It was found that the LNA bases in a MB stem sequence had a significant effect on the stability of the hair-pin structure. The hybridization rates of LNA-MBs were significantly improved by lowering the DNA/LNA ratio in the probe, and most significantly, by having a shared-stem design for the LNA-MB to prevent sticky-end pairing. It was found that only MB sequences with DNA/LNA alternating bases or all LNA bases were able to resist nonspecific protein binding and DNase I digestion. Additional results showed that a sequence consisting of a DNA stretch less than three bases between LNA bases was able to block RNase H function. This study suggested that a shared-stem MB with a 4 base-pair stem and alternating DNA/LNA bases is desirable for intracellular applications as it ensures reasonable hybridization rates, reduces protein binding and resists nuclease degradation for both target and probes. These findings have implications on the design of LNA molecular probes for intracellular monitoring application, disease diagnosis and basic biological studies.
语种	英语
内容类型	期刊论文
源URL	[http://dspace.xmu.edu.cn/handle/2288/62402]
专题	化学化工－已发表论文
推荐引用方式 GB/T 7714	Yang CY. Synthesis and investigation of deoxyribonucleic acid/locked nucleic acid chimeric molecular beacons[J],2007.
APA	杨朝勇.(2007).Synthesis and investigation of deoxyribonucleic acid/locked nucleic acid chimeric molecular beacons..
MLA	杨朝勇."Synthesis and investigation of deoxyribonucleic acid/locked nucleic acid chimeric molecular beacons".(2007).

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