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	Characterization of mercurial-bound metallothioneins in rat kidney by hyphenated techniques
	Zhuang,ZX ; Shen,JC ; Huang,ZY ; Li,XC ; Wang,XR ; Zhuang ZS(庄峙厦)
	2005-01
关键词	REVERSED-PHASE HPLC MASS-SPECTROMETRIC DETECTION ISOFORMS ELECTROPHORESIS IDENTIFICATION
英文摘要	Multimetal-bound metallothioneins (MT) induced in kidney of rat after oral intake of HgCl2 were characterized with hyphenated techniques as size-exclusion chromatography (SEC) coupled with inductively coupled plasma mass spectrometry (ICP-MS) and reverse phase chromatography (RPC) coupled with electrospray ionization mass spectrometry (EST-MS). The identification of MT fractions in the size-exclusion chromatographic profiles was achieved by both of the chromatogram of SEC-ICP-MS and the corresponding UV spectra of MT. The amount of Hg-MT in rat kidney of HgCl2 group significantly increased compared with that of the control group. In addition, the signal of Cu-MT was largely induced in polluted rat tissue. The results validated the important roles of MT played in the homeostasis of Cu for the detoxification of mercury. After being purified with size-exclusion column (Sephadex G-75) and dialysis (M<1000 Da), the extracts of rat kidney was separated with a narrow-bore reverse phase column and eluted by increasing methanol at gradient mode. The MT polymorphism with different binding-metal (including Hg, Cu and Zn) species was observed by ESI-MS. The tentative formulas of Hg-MT isoforms and subisoforms were interpreted by the measured masses at the vicinity of apex in total Ion current (TIC) of ESI-MS and the reference data of apo-MT reported. The results indicated that many Hg-binding MT species existed in the polluted rat kidney.
语种	英语
内容类型	期刊论文
源URL	[http://dspace.xmu.edu.cn/handle/2288/61806]
专题	化学化工－已发表论文
推荐引用方式 GB/T 7714	Zhuang,ZX,Shen,JC,Huang,ZY,et al. Characterization of mercurial-bound metallothioneins in rat kidney by hyphenated techniques[J],2005.
APA	Zhuang,ZX,Shen,JC,Huang,ZY,Li,XC,Wang,XR,&庄峙厦.(2005).Characterization of mercurial-bound metallothioneins in rat kidney by hyphenated techniques..
MLA	Zhuang,ZX,et al."Characterization of mercurial-bound metallothioneins in rat kidney by hyphenated techniques".(2005).

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