QCM and EC-SPR Studies of Cytochrome c Self-assembled on Au Electrode and Enhancement of SPR Signal by Au Nanoparticles | |
Wang, Y. Y. ; Jiang, Y. X. ; Zhou, Y. C. ; Li, Y. Y. ; Ma, Z. F. ; Sun, S. G. ; Sun SG(孙世刚) | |
2012-11 | |
关键词 | SURFACE-PLASMON RESONANCE DIRECT ELECTROCHEMISTRY CONFORMATIONAL-CHANGES GOLD SUBSTRATE PROTEIN HYBRIDIZATION VOLTAMMETRY MONOLAYERS SYSTEMS FILMS |
英文摘要 | National Natural Science Foundation of China [20833005, 20873116, 60936003, 21021002]; Fund of the State Key Laboratory of Superamolecular Structure and Materials, Jilin University, China [200910]; Quartz crystal microbalance(QCM) and cyclic voltammetry(CV) were used to characterize the monolayer of cytochrome c(Cyt c), which was adsorbed on gold film modified with alkanethiol mixed monolayer. A direct comparison of protein surface coverages calculated from QCM and cyclic voltammetric measurements illustrates that the ratio of the electroactive Cyt c to the total surface-confined Cyt c is 34%, which suggests that the orientation is a main factor affecting the electroactivity of Cyt c. Moreover, surface plasmon resonance(SPR) measurement combined with CV "in situ" was used to investigate the conformational change of Cyt c in the redox process. Besides, Au nanoparticles(Au NPs) were adsorbed on the surface of Cyt c. The result indicates that Au NPs promote electron transfer between Cyt c and the gold electrode, and SPR result suggests Au NPs enhance SPR signal. |
语种 | 英语 |
内容类型 | 期刊论文 |
源URL | [http://dspace.xmu.edu.cn/handle/2288/61701] |
专题 | 化学化工-已发表论文 |
推荐引用方式 GB/T 7714 | Wang, Y. Y.,Jiang, Y. X.,Zhou, Y. C.,et al. QCM and EC-SPR Studies of Cytochrome c Self-assembled on Au Electrode and Enhancement of SPR Signal by Au Nanoparticles[J],2012. |
APA | Wang, Y. Y..,Jiang, Y. X..,Zhou, Y. C..,Li, Y. Y..,Ma, Z. F..,...&孙世刚.(2012).QCM and EC-SPR Studies of Cytochrome c Self-assembled on Au Electrode and Enhancement of SPR Signal by Au Nanoparticles.. |
MLA | Wang, Y. Y.,et al."QCM and EC-SPR Studies of Cytochrome c Self-assembled on Au Electrode and Enhancement of SPR Signal by Au Nanoparticles".(2012). |
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