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	An integrated electrochemical device based on immunochromatographic test strip and enzyme labels for sensitive detection of disease-related biomarkers
	Zou, Zhe-Xiang ; Wang, Jun ; Wang, Hua ; Li, Yao-Qun ; Li YQ(李耀群) ; Lin, Yuehe
	2012-05-30
关键词	Immunochromarographic test strip assay Horseradish peroxidase Hepatitis B surface antigen Disease-related biomarker
英文摘要	A novel electrochemical biosensing device that integrates an immunochromatographic test strip and a screen-printed electrode (SPE) connected to a portable electrochemical analyzer was presented for rapid, sensitive, and quantitative detection of disease-related biomarker in human blood samples. The principle of the sensor is based on sandwich immunoreactions between a biomarker and a pair of its antibodies on the test strip, followed by highly sensitive square-wave voltammetry (SWV) detection. Horseradish peroxidase (HRP) was used as a signal reporter for electrochemical readout. Hepatitis B surface antigen (HBsAg) was employed as a model protein biomarker to demonstrate the analytical performance of the sensor in this study. Some critical parameters governing the performance of the sensor were investigated in detail. Under optimal conditions, this sensor was capable of detecting a minimum of 0.3 ng mL(-1) (S/N = 3) HBsAg with a wide linear concentration range from 1 to 500 ng mL-1. The sensor was further utilized to detect HBsAg spiked in human plasma with an average recovery of 91.3%. In comparison, a colorimetric immunochromatographic test strip assay (ITSA) was also conducted. The result shows that the SWV detection in the electrochemical sensor is much more sensitive for the quantitative determination of HBsAg than the colorimetric detection, indicating that such a sensor is a promising platform for rapid and sensitive point-of-care testing/screening of disease-related biomarkers in a large population. (C) 2012 Elsevier B.V. All rights reserved.; National Institute of Environmental Health Sciences (NIEHS), NIH [U54 OH008173-01]; National Institutes of Health Counter through National Institute of Neurological Disorders and Stroke, NIH [U01 NS058161-01]; National Natural Science Foundation of China [20975084]; DOE by Battelle [DE-AC05-76L01830]; China Scholarship Council; PNNL
语种	英语
出版者	ELSEVIER SCIENCE BV
内容类型	期刊论文
源URL	[http://dx.doi.org/10.1016/j.talanta.2012.02.046]
专题	化学化工－已发表论文
推荐引用方式 GB/T 7714	Zou, Zhe-Xiang,Wang, Jun,Wang, Hua,et al. An integrated electrochemical device based on immunochromatographic test strip and enzyme labels for sensitive detection of disease-related biomarkers[J],2012.
APA	Zou, Zhe-Xiang,Wang, Jun,Wang, Hua,Li, Yao-Qun,李耀群,&Lin, Yuehe.(2012).An integrated electrochemical device based on immunochromatographic test strip and enzyme labels for sensitive detection of disease-related biomarkers..
MLA	Zou, Zhe-Xiang,et al."An integrated electrochemical device based on immunochromatographic test strip and enzyme labels for sensitive detection of disease-related biomarkers".(2012).

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