A kinetic and thermodynamic study of the lipase-catalyzed remote resolution of a chiral tertiary alcohol | |
Wang, Shi-Zhen ; Wang SZ(王世珍) ; Wu, Jian-Ping ; Xu, Gang ; Yang, Li-Rong | |
2012-06-15 | |
关键词 | Dynamic modeling Kinetic parameters Lipase Immobilized enzymes Tertiary alcohol Asymmetric alcoholysis |
英文摘要 | The kinetics and thermodynamics of Candida antarctica lipase B-catalyzed remote asymmetric alcoholysis of the citalopram intermediate 3-[(acetyloxy)methyl]-4-[4-(dimethylamino)-1-(4-fluorophenyl)-1-hydroxybutyl]-benzonitrile (diol monoacetate) have been studied. A kinetic model based on the reversible ping-pong bi-bi mechanism with competitive enantiomer substrates was proposed. The product inhibition by each diol enantiomer and the substrate inhibition by isobutyl alcohol were also considered. The diffusion limitation was proven to be negligible. By reducing the degree of freedom in parameter estimation by model discrimination and Haldane equations, 14 free parameters were successfully identified. The model parameters were simulated by the Matlab program using time-concentration curves of different diol monoacetate concentrations; the simulated values fit the experimental values well, with an average relative error of 9.6%. The reaction activity and enantioselectivity of C. antarctica lipase B toward the tertiary alcohol were investigated by kinetic and thermodynamic analysis using simulated kinetic parameters. (c) 2012 Elsevier B.V. All rights reserved.; National Natural Science Foundation of China [20936002]; Key Project of Chinese National Programs for Fundamental Research and Development [2011CB710800]; Hi-Tech Research and Development Program of China [2011AA02A209] |
语种 | 英语 |
出版者 | ELSEVIER SCIENCE SA |
内容类型 | 期刊论文 |
源URL | [http://dx.doi.org/10.1016/j.bej.2012.04.005] |
专题 | 化学化工-已发表论文 |
推荐引用方式 GB/T 7714 | Wang, Shi-Zhen,Wang SZ,Wu, Jian-Ping,et al. A kinetic and thermodynamic study of the lipase-catalyzed remote resolution of a chiral tertiary alcohol[J],2012. |
APA | Wang, Shi-Zhen,王世珍,Wu, Jian-Ping,Xu, Gang,&Yang, Li-Rong.(2012).A kinetic and thermodynamic study of the lipase-catalyzed remote resolution of a chiral tertiary alcohol.. |
MLA | Wang, Shi-Zhen,et al."A kinetic and thermodynamic study of the lipase-catalyzed remote resolution of a chiral tertiary alcohol".(2012). |
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