QQ客服 官方微博 反馈留言

	Selection of DNA Aptamers against Glioblastoma Cells with High Affinity and Specificity
	Kang, Dezhi ; Wang, Jiangjie ; Zhang, Weiyun ; Song, Yanling ; Li, Xilan ; Zou, Yuan ; Zhu, Mingtao ; Zhu, Zhi ; Chen, Fuyong ; Yang, Chaoyong James ; Yang CY(杨朝勇)
	2012-10-02
关键词	NUCLEIC-ACID APTAMERS CANCER-CELLS MOLECULAR RECOGNITION SYSTEMATIC EVOLUTION BRAIN-TUMORS LIVE CELLS SELEX STRATEGIES SURVIVAL THERAPY
英文摘要	Background: Glioblastoma is the most common and most lethal form of brain tumor in human. Unfortunately, there is still no effective therapy to this fatal disease and the median survival is generally less than one year from the time of diagnosis. Discovery of ligands that can bind specifically to this type of tumor cells will be of great significance to develop early molecular imaging, targeted delivery and guided surgery methods to battle this type of brain tumor. Methodology/Principal Findings: We discovered two target-specific aptamers named GBM128 and GBM131 against cultured human glioblastoma cell line U118-MG after 30 rounds selection by a method called cell-based Systematic Evolution of Ligands by EXponential enrichment (cell-SELEX). These two aptamers have high affinity and specificity against target glioblastoma cells. They neither recognize normal astraglial cells, nor do they recognize other normal and cancer cell lines tested. Clinical tissues were also tested and the results showed that these two aptamers can bind to different clinical glioma tissues but not normal brain tissues. More importantly, binding affinity and selectivity of these two aptamers were retained in complicated biological environment. Conclusion/Significance: The selected aptamers could be used to identify specific glioblastoma biomarkers. Methods of molecular imaging, targeted drug delivery, ligand guided surgery can be further developed based on these ligands for early detection, targeted therapy, and guided surgery of glioblastoma leading to effective treatment of glioblastoma.; National Basic Research Program of China [2010CB732402]; National Instrumentation Program [2011YQ03012412]; Natural Science Foundation of Fujian Province for Distinguished Young Scholars [2010 J06004]; Key Laboratory for Chemical Biology of Fujian Province
语种	英语
出版者	PUBLIC LIBRARY SCIENCE
内容类型	期刊论文
源URL	[http://dx.doi.org/10.1371/journal.pone.0042731]
专题	化学化工－已发表论文
推荐引用方式 GB/T 7714	Kang, Dezhi,Wang, Jiangjie,Zhang, Weiyun,et al. Selection of DNA Aptamers against Glioblastoma Cells with High Affinity and Specificity[J],2012.
APA	Kang, Dezhi.,Wang, Jiangjie.,Zhang, Weiyun.,Song, Yanling.,Li, Xilan.,...&杨朝勇.(2012).Selection of DNA Aptamers against Glioblastoma Cells with High Affinity and Specificity..
MLA	Kang, Dezhi,et al."Selection of DNA Aptamers against Glioblastoma Cells with High Affinity and Specificity".(2012).

个性服务

查看访问统计

相关权益政策

暂无数据

收藏/分享

除非特别说明，本系统中所有内容都受版权保护，并保留所有权利。