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	Study on fluorometric determination of hydrogen peroxide catalyzed by iron(III)-tetrasulfonato-phthalocyanine with thiamine hydrochloride as a substrate
	Chen, QY ; Li, DH ; Li DH(李道华) ; Zhu, QZ ; Zheng, H ; Xu, JG ; Xu JG(许金钩)
	2008-02-18
关键词	mimetic enzyme spectrofluorimetric determination hydrogen peroxide glucose thiamine iron-tetrasulfonatophthalocyanine
英文摘要	Iron(III)-tetrasulfonatophthalocyanine(FeTSPc) has been used as a mimetic enzyme in the determination of hydrogen peroxide with thiamine hydrochloride as a fluorogenic substrate. The determinations were carried out in both acidic and basic environments, with different limits of detection and linear ranges. In acidic condition, the linear calibration graph was obtained from 5.0x10(-8) mol/L to 8.0x10(-6) mol/L, with a detection limit of 2.1x10(-8) mol/L H2O2 when Na2HPO4-citric buffer solution (pH 2.8) was used as the reaction medium. It was also found that using one of the three polybasic carboxylic acids such as citric acid, tartaric acid and malonic acid as the catalytic reaction medium can lead to particularly sensitive systems, permitting a detection limit as low as 3.5x10(-9) mol/L H2O2; whereas in basic reaction medium (Na2CO3-NaHCO3 buffer solution, pH = 10.0), the linear range of the calibration graph was from 5.0x10(-8) mol/L to 2.0x10(6) mol/L H2O2 with a detection limit of 1.4x10(-8) mol/L. The applicability of the method to the determination of glucose in human serum was demonstrated by investigating the recovery of the known glucose added to human serum.
语种	英语
出版者	MARCEL DEKKER INC
内容类型	期刊论文
源URL	[http://dx.doi.org/doi:10.1080/00032719908542833]
专题	化学化工－已发表论文
推荐引用方式 GB/T 7714	Chen, QY,Li, DH,Li DH,et al. Study on fluorometric determination of hydrogen peroxide catalyzed by iron(III)-tetrasulfonato-phthalocyanine with thiamine hydrochloride as a substrate[J],2008.
APA	Chen, QY.,Li, DH.,李道华.,Zhu, QZ.,Zheng, H.,...&许金钩.(2008).Study on fluorometric determination of hydrogen peroxide catalyzed by iron(III)-tetrasulfonato-phthalocyanine with thiamine hydrochloride as a substrate..
MLA	Chen, QY,et al."Study on fluorometric determination of hydrogen peroxide catalyzed by iron(III)-tetrasulfonato-phthalocyanine with thiamine hydrochloride as a substrate".(2008).

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