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	Brilliant cresyl blue as a new red region fluorescent probe for determination of nucleic acids
	Zheng, H ; Chen, XL ; Zhu, CQ ; Li, DH ; Chen, QY ; Xu, JG ; Xu JG(许金钩)
	2000-04-26
关键词	red region fluorescence nucleic acids fluorometric method brilliant cresyl blue (BCB)
英文摘要	A fluorescence quenching method was developed for determination of microamounts of nucleic acids by using brilliant cresyl blue (BCB) as a new red region fluorescent probe. In aqueous hexylmethylene tetramine solution, BCB showed maximum excitation and emission wavelengths at 626 and 670 nm, respectively, and the fluorescence of BCB could be greatly quenched by DNA (or RNA). Under optimal conditions, the calibration graphs are linear over the range of 0.02-0.80 mu g/ml for SM DNA and 0.25-1.5 mu g/ml for yeast RNA. The corresponding detection limits are 7 ng/ml for SM DNA and 25 ng/ml for yeast RNA, respectively. SM DNA can be determinated in the presence of 40% (w/w) RNA, and the relative standard deviation of six measurements is 2.5% for 500 ng/ml SM DNA. The result of the determination of golden staphylococcus DNA by this method was satisfactory. (C) 2000 Elsevier Science B.V. All rights reserved.
语种	英语
出版者	ELSEVIER SCIENCE BV
内容类型	期刊论文
源URL	[http://dx.doi.org/10.1016/S0026-265X(00)00015-1]
专题	化学化工－已发表论文
推荐引用方式 GB/T 7714	Zheng, H,Chen, XL,Zhu, CQ,et al. Brilliant cresyl blue as a new red region fluorescent probe for determination of nucleic acids[J],2000.
APA	Zheng, H.,Chen, XL.,Zhu, CQ.,Li, DH.,Chen, QY.,...&许金钩.(2000).Brilliant cresyl blue as a new red region fluorescent probe for determination of nucleic acids..
MLA	Zheng, H,et al."Brilliant cresyl blue as a new red region fluorescent probe for determination of nucleic acids".(2000).

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