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	Determination of nucleic acids based on shifting the association equilibrium between tetrasulfonated aluminium phthalocyanine and Acridine Orange
	Li, DH ; Chen, XL ; Chen XL(陈小兰) ; Fang, Y ; Xu, JG ; Xu JG(许金钩) ; Li DH(李东辉)
	2001-02-06
英文摘要	Based on the ability of nucleic acids to shift the association equilibrium of the ion-association complex of Acridine Grant and tetrasulfonated aluminium phthalocyanine, thus leading to an increase in the phthalocyanine fluorescence, a method is suggested for the fluorimetric determination of nucleic acids. Investigations were carried out on the spectral characteristics, order of addition of reagents, selection of the buffer system, effect of pH, influence of reaction time, effect of salt, the usage of reagents, interference of foreign substances and the effect of different acridine derivatives. Under the optimum conditions, the calibration graphs for the determination of calf thymus DNA (CT DNA). salmon DNA (SM DNA) and yeast RNA were linear over the ranges 0.04-1.2, 0.04-1.2 and 0.1-1.2 mug cm(-1), respectively. The detection limits for CT DNA, SM DNA and RNA were 17, 24 and 98 mug cm(-3), respectively. The relative standard deviation (n = 6) was within 4.6% for the detection of samples. The method was applied to the determination bf Staphylococcus aureus DNA and the result was in agreement with that achieved by a UV method.
语种	英语
出版者	ROYAL SOC CHEMISTRY
内容类型	期刊论文
源URL	[http://dx.doi.org/doi:10.1039/B008997O]
专题	化学化工－已发表论文
推荐引用方式 GB/T 7714	Li, DH,Chen, XL,Chen XL,et al. Determination of nucleic acids based on shifting the association equilibrium between tetrasulfonated aluminium phthalocyanine and Acridine Orange[J],2001.
APA	Li, DH.,Chen, XL.,陈小兰.,Fang, Y.,Xu, JG.,...&李东辉.(2001).Determination of nucleic acids based on shifting the association equilibrium between tetrasulfonated aluminium phthalocyanine and Acridine Orange..
MLA	Li, DH,et al."Determination of nucleic acids based on shifting the association equilibrium between tetrasulfonated aluminium phthalocyanine and Acridine Orange".(2001).

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