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	Enzyme-linked fluorescence immunoassay for human IgG by using a pH-sensitive phase separating polymer as carrier
	Lin, P ; Lin P(林鹏) ; Zheng, H ; Zheng H(郑洪) ; Yang, HH ; Yang HH(杨黄浩) ; Yang, W ; Yang W(杨薇) ; Zhang, CG ; Zhang ZG(张长弓) ; Xu, JG ; Xu JG(许金钩)
	2003
关键词	pH-sensitive phase separating polymer enzyme-linked fluorescence immunoassay human IgG
英文摘要	Poly (N-isopropylacrylamide-N, N'-dimethyl aminopropyl methacrylamide) [P(NIP-DMAPM)], a novel linear water-soluble pH-sensitive polymer whose phase transition pH is about 7.4 at 37 degreesC, was synthesized. P(NIP-DMAPM) polymer precipitated out of water above a critical pH 7.4 at 37. degreesC and redissolved at pH below 7.4. P (NIP-DMAPM) was used as a novel separation carrier for the reactants in immunoassay and a enzyme-linked fluorescence immunoassay for human IgG was built. The, linear, range of determination was 0similar to1700 ng/mL. and the, limit of detection was 2.2 ng/mL. The sensitivity, of this method was close to that of traditional ELISA by using same reactants. However, the assay was much faster. This method has,been applied to the determination of the human IgG levels in human serum with satisfactory results.
语种	中文
出版者	HIGHER EDUCATION PRESS
内容类型	期刊论文
源URL	[http://dspace.xmu.edu.cn/handle/2288/12131]
专题	化学化工－已发表论文
推荐引用方式 GB/T 7714	Lin, P,Lin P,Zheng, H,et al. Enzyme-linked fluorescence immunoassay for human IgG by using a pH-sensitive phase separating polymer as carrier[J],2003.
APA	Lin, P.,林鹏.,Zheng, H.,郑洪.,Yang, HH.,...&许金钩.(2003).Enzyme-linked fluorescence immunoassay for human IgG by using a pH-sensitive phase separating polymer as carrier..
MLA	Lin, P,et al."Enzyme-linked fluorescence immunoassay for human IgG by using a pH-sensitive phase separating polymer as carrier".(2003).

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