Differential protein expression of kidney tissue in the scallop Patinopecten yessoensis under acute cadmium stress | |
Huang, Xiang ; Fang, Cai-Wang ; Guo, Yan-Wei ; Huang, He-Qing ; Huang HQ(黄河清) | |
2011-04-04 | |
关键词 | Cadmium Proteomics MALDI Protein biomarker Scallop Kidney |
英文摘要 | Morphological and proteomic changes in the kidney of scallops exposed to acute cadmium chloride (CdCl(2)) were observed, analyzed and compared with those in the non-exposed control group. Under microscopy the paraffin-embedded sections of the kidney revealed that the microstructure of the tissue had been severely deformed after Cd exposure. Two dimensional electrophoresis, MALDI-TOF mass spectrometry and database searches showed 13 differentially expressed protein spots, of which 11 were up-regulated, while two were down-regulated. Among these proteins, guanylate kinase (GK) and C(2)H(2)-type zinc finger protein are considered to be tightly connected with Cd toxicity. Further studies using quantitative PCR method validated that the GK mRNA was induced under Cd stress. Other proteins identified which had some relevance to Cd toxicity are also discussed. We suggested that differential proteins such as GK could play a potential role as novel biomarkers for monitoring the level of Cd contamination in seawater. (C) 2011 Elsevier Inc. All rights reserved.; State Natural Science Fund[30870515, 40776060]; 973 Projects[2010CB126403]; PCSIRT Project, China[IRT0941] |
语种 | 英语 |
出版者 | ACADEMIC PRESS INC ELSEVIER SCIENCE |
内容类型 | 期刊论文 |
源URL | [http://dx.doi.org/doi:10.1016/j.ecoenv.2011.02.005] ![]() |
专题 | 化学化工-已发表论文 |
推荐引用方式 GB/T 7714 | Huang, Xiang,Fang, Cai-Wang,Guo, Yan-Wei,et al. Differential protein expression of kidney tissue in the scallop Patinopecten yessoensis under acute cadmium stress[J],2011. |
APA | Huang, Xiang,Fang, Cai-Wang,Guo, Yan-Wei,Huang, He-Qing,&黄河清.(2011).Differential protein expression of kidney tissue in the scallop Patinopecten yessoensis under acute cadmium stress.. |
MLA | Huang, Xiang,et al."Differential protein expression of kidney tissue in the scallop Patinopecten yessoensis under acute cadmium stress".(2011). |
个性服务 |
查看访问统计 |
相关权益政策 |
暂无数据 |
收藏/分享 |
除非特别说明,本系统中所有内容都受版权保护,并保留所有权利。
修改评论