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	Biogenic Silver Nanoparticles by Cacumen Platycladi Extract: Synthesis, Formation Mechanism, and Antibacterial Activity
	Huang, Jiale ; Huang JL(黄加乐) ; Zhan, Guowu ; Zheng, Bingyun ; Sun, Daohua ; Sun DH(孙道华) ; Lu, Fenfen ; Lin, Yuan ; Chen, Huimei ; Zheng, Zhouding ; Zheng, Yanmei ; Li, Qingbiao ; Li QB(李清彪)
	2011-06-21
英文摘要	Biosynthesis of Ag nanoparticles (AgNPs) by Cacumen Platycladi extract was investigated. The AgNPs were characterized by ultraviolet visible absorption spectroscopy (UV-vis), transmission electron microscopy (TEM), selected-area electron diffraction (SAED), and X-ray diffraction (XRD). The results showed that increasing the initial AgNO(3) concentration at 30 or 60 degrees C increased the mean size and widened the size distribution of the AgNPs leading to red shift and broadening of the Surface Plasmon Resonance absorption. The conversion of silver ions was determined by atomic absorption spectroscopy (AAS) and to discuss the bioreductive mechanism, the reducing sugar, flavonoid, saccharide, protein contents in the extract, and the antioxidant activity were measured using 3,5-dinitrosalicylic acid colorimetric; Coomassie brilliant blue; phenol-sulfuric acid; rutin-based spectrophotometry method; and 2,2-diphenyl-1-picrylhydrazyl (DPPH) free radical-scavenging assay methods. The results showed that the reducing sugars and flavonoids were mainly responsible for the bioreduction of the silver ions and their reductive capability promoted at 90 degrees C, leading to the formation of AgNPs (18.4 +/- 4.6 nm) with narrow size distribution. Finally, the antibacterial activity of the AgNPs against E. coli and S. aureus was assessed to determine their potential applications in silver-loaded antibacterial materials. This work provides useful technical parameters for industrialization of the biosynthetic technique and further antibacterial application of the AgNPs. Furthermore, the elucidation of bioreductive mechanism of silver ions by measuring the change of the biomolecular concentrations in plant extract exemplifies understanding the formation mechanism of such biogenic AgNPs.; national Natural Science Foundation of China[21036004, 20776120, 20976146]; national 863 Program of China[2007AA03Z347]; Natural Science Foundation of Fujian Province of China[2010J05032, 2010J01052]; Fundamental Research Funds for Central Universities[2010121051]
语种	英语
出版者	AMER CHEMICAL SOC
内容类型	期刊论文
源URL	[http://dx.doi.org/doi:10.1021/ie200858y]
专题	化学化工－已发表论文
推荐引用方式 GB/T 7714	Huang, Jiale,Huang JL,Zhan, Guowu,et al. Biogenic Silver Nanoparticles by Cacumen Platycladi Extract: Synthesis, Formation Mechanism, and Antibacterial Activity[J],2011.
APA	Huang, Jiale.,黄加乐.,Zhan, Guowu.,Zheng, Bingyun.,Sun, Daohua.,...&李清彪.(2011).Biogenic Silver Nanoparticles by Cacumen Platycladi Extract: Synthesis, Formation Mechanism, and Antibacterial Activity..
MLA	Huang, Jiale,et al."Biogenic Silver Nanoparticles by Cacumen Platycladi Extract: Synthesis, Formation Mechanism, and Antibacterial Activity".(2011).

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