QQ客服 官方微博 反馈留言

	Linear molecular beacons for highly sensitive bioanalysis based on cyclic Exo III enzymatic amplification
	Yang, Chaoyong James ; Yang CY(杨朝勇) ; Cui, Liang ; Huang, Jiahao ; Yan, Ling ; Lin, Xiaoyan ; Wang, Chunming ; Zhang, WeiYun ; Kang, Huaizhi
	2011-06-28
关键词	Cocaine DNA Exo III Linear molecular beacons Signal amplification
英文摘要	Sensitive analysis or monitoring of biomolecules and small molecules is very important for many biological researches, clinical diagnosis and forensic investigations. As a sequence-independent exonuclease, Exonuclease III (Exo III) has been widely used for amplified detection of proteins and nucleic acids where displacing probes or molecular beacons are used as the signaling probes. However, displacing probes suffer slow hybridization rate and high background signal and molecular beacons are difficult to design and prone to undesired nonspecific interactions. Herein, we report a new type of probes called linear molecular beacons (LMBs) for use in Exo III amplification assays to improve hybridization kinetics and reduce background noises. LMBs are linear oligonucleotide probes with a fluorophore and quencher attached to 3' terminal and penultimate nucleotides, respectively. Compared to conventional molecular beacons and displacing probes, LMBs are easy to design and synthesize. More importantly, LMBs have a much lower background noise and allow faster reaction rates. Using LMBs in cyclic Exo III amplification assay, ultrasensitive nucleic acid detection methods were developed with a detection limit of less than 120 fM, which is 2 orders of magnitude lower than that of conventional molecular beacons or displacing probes-based Exo III amplification assays. Furthermore, LMBs can be extended as universal probes for detection of non-nucleic acid molecules such as cocaine with high sensitivity. These results demonstrate that the combination of Exo III amplification and LMB signaling provides a general method for ultrasensitive and selective detection of a wide range of targets. (C) 2011 Elsevier B.V. All rights reserved.; Natural Science Foundation of Fujian Province, China[2008J0107]; Natural Science Foundation of Fujian Province for Distinguished Young Scholar[2010J06004]; National Science Foundation of China[20805038]; 973 Program of China[2007CB935603, 2010CB732402]
语种	英语
出版者	ELSEVIER ADVANCED TECHNOLOGY
内容类型	期刊论文
源URL	[http://dx.doi.org/doi:10.1016/j.bios.2011.06.027]
专题	化学化工－已发表论文
推荐引用方式 GB/T 7714	Yang, Chaoyong James,Yang CY,Cui, Liang,et al. Linear molecular beacons for highly sensitive bioanalysis based on cyclic Exo III enzymatic amplification[J],2011.
APA	Yang, Chaoyong James.,杨朝勇.,Cui, Liang.,Huang, Jiahao.,Yan, Ling.,...&Kang, Huaizhi.(2011).Linear molecular beacons for highly sensitive bioanalysis based on cyclic Exo III enzymatic amplification..
MLA	Yang, Chaoyong James,et al."Linear molecular beacons for highly sensitive bioanalysis based on cyclic Exo III enzymatic amplification".(2011).

个性服务

查看访问统计

相关权益政策

暂无数据

收藏/分享

除非特别说明，本系统中所有内容都受版权保护，并保留所有权利。