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	Soluble Fusion Expression and Characterization of Human Beta-defensin 3 Using a Novel Approach
	Dong, Jing（ China Pharmaceut Univ, Sch Life Sci & Technol） ; Yu, Heguo（Shanghai Inst Planned Parenthood Res） ; Zhang, Yonglian（Shanghai Inst Planned Parenthood Res） ; Diao, Hua（Shanghai Inst Planned Parenthood Res） ; Lin, Donghai ; Lin DH(林东海)
	2011
关键词	Expression purification intein human beta-defensin3 antimicrobial activity
英文摘要	Human beta-defensin 3 (DEFB103) is a recently identified small cysteine-rich cationic peptide expressed ubiquitously upon local microbial invasion. A number of accumulating evidences indicate that this peptide is involved in many biological processes, including microbicidal activities, chemoattraction, and immunomodulation. In this article, we describe a novel approach through which we performed the expression and purification of the recombinant DEFB103 peptide in Escherichia coli (E. coli) based on the pTWIN1 expression system. This approach does not introduce any extra residues to the peptide product, and also eliminates the requirement of removing the fusion tag by exogenous proteases. A high yield of 112 mg of soluble fusion DEFB103 was obtained in 1 liter of Luria-Bertani (LB) medium. By one-step affinity chromatography and on-column, auto-cleavage of the fusion tag, the mature DEFB103 peptide was produced with a yield of 30 mg/L LB. The purified DEFB103 peptide demonstrated strong antimicrobial activities against E. coli, S. aureus and C. albicans, which were representatives of Gram-negative and Gram-positive bacteria and fungi, respectively. Using this novel approach, we have successfully expressed and purified several human defensins with significant bioactivities. Our work may be helpful for structural and functional studies of other human defensins, and also for the production of human defensins.; Program of Shanghai Subject Chief Scientist[09XD1405100]
语种	英语
出版者	BENTHAM SCIENCE PUBL LTD
内容类型	期刊论文
源URL	[http://dspace.xmu.edu.cn/handle/2288/11464]
专题	化学化工－已发表论文
推荐引用方式 GB/T 7714	Dong, Jing,Yu, Heguo,Zhang, Yonglian,et al. Soluble Fusion Expression and Characterization of Human Beta-defensin 3 Using a Novel Approach[J],2011.
APA	Dong, Jing,Yu, Heguo,Zhang, Yonglian,Diao, Hua,Lin, Donghai,&林东海.(2011).Soluble Fusion Expression and Characterization of Human Beta-defensin 3 Using a Novel Approach..
MLA	Dong, Jing,et al."Soluble Fusion Expression and Characterization of Human Beta-defensin 3 Using a Novel Approach".(2011).

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