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	Determination of nucleic acids based on shifting the association equilibrium between tetracarboxy aluminum phthalocyanine and poly-lysine
	Changqing Zhu ; Shujuan Zhuo ; Hong Zheng ; Zheng H(郑洪) ; Jinlong Chen ; Donghui Li ; Shunhua Li ; Li SH(李顺华) ; Jingou Xu ; Xu JG(许金钩)
	2004-08
关键词	near-IR fluorescence tetracarboxy aluminum phthalocyanine poly-lysine nucleic acids
英文摘要	A new method based on near-infrared (near-IR) fluorescence recovery was presented for the determination of nucleic acids. This method employed a two-regent system composed of anionic tetracarboxy aluminum phthalocyanine, (AlC4Pc) and polycationic poly-lysine. The fluorescence of AlC4Pc, with the maximum excitation and emission wavelengths at 620 and 701 nm, respectively, was quenched by polylysine with a proper concentration, but recovered by adding nucleic acids. Under optimal conditions, the recovered fluorescence was in proportional to the concentration of nucleic acids. The linear ranges of the calibration curves were 5-200 ng mL(-1) for both calf thymus DNA (ctDNA) and fish sperm DNA (fsDNA) with the detection limit of 2.6 ng mL(-1) for ctDNA and 2.1 ng mL(-1) for fsDNA. The relative standard deviation (n = 6) was 1.9 and 1.3% for 50 ng mL(-1) ctDNA and fsDNA, respectively. The proposed method was applied to the determination of nucleic acids in synthetic samples with satisfactory results. (C) 2004 Elsevier B.V. All rights reserved.
语种	英语
出版者	PERGAMON-ELSEVIER SCIENCE LTD
内容类型	期刊论文
源URL	[http://dx.doi.org/doi:10.1016/j.saa.2004.05.029]
专题	化学化工－已发表论文
推荐引用方式 GB/T 7714	Changqing Zhu,Shujuan Zhuo,Hong Zheng,et al. Determination of nucleic acids based on shifting the association equilibrium between tetracarboxy aluminum phthalocyanine and poly-lysine[J],2004.
APA	Changqing Zhu.,Shujuan Zhuo.,Hong Zheng.,郑洪.,Jinlong Chen.,...&许金钩.(2004).Determination of nucleic acids based on shifting the association equilibrium between tetracarboxy aluminum phthalocyanine and poly-lysine..
MLA	Changqing Zhu,et al."Determination of nucleic acids based on shifting the association equilibrium between tetracarboxy aluminum phthalocyanine and poly-lysine".(2004).

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