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	Determination of alkali-labile phosphoprotein phosphorus from fish plasma using the Tb3+-tiron complex as a fluorescence probe
	Lv Xue-fei ; Zhao Yi-bing ; Zhao YB(赵一兵) ; Zhou Qun-fang ; Jiang Gui-bin ; Song Mao-yong
	2007-06
关键词	alkali-labile phosphoprotein phosphorus (ALP) Chinese loach fluorescence quenching Tb3+-Tiron complex vitellogenin
英文摘要	A sensitive method based on the fluorescence quenching effect of the Tb3+-Tiron complex is proposed for the determination of alkali-labile phosphoprotein phosphorus (ALP) released from fish plasma. The detection limit was 5.4 ng/ml (S/N=2), and the relative standard deviation of the quenching effect (6 replicates) was 4.6%. The results obtained by the proposed method were in good agreement with those obtained by the colorimetric assay. The advantages of the present method are its relatively simple detection procedure, the lack of toxic organic solvents, and high sensitivity.
语种	英语
出版者	SCIENCE CHINA PRESS
内容类型	期刊论文
源URL	[http://dx.doi.org/doi:10.1016/S1001-0742(07)60102-8]
专题	化学化工－已发表论文
推荐引用方式 GB/T 7714	Lv Xue-fei,Zhao Yi-bing,Zhao YB,et al. Determination of alkali-labile phosphoprotein phosphorus from fish plasma using the Tb3+-tiron complex as a fluorescence probe[J],2007.
APA	Lv Xue-fei,Zhao Yi-bing,赵一兵,Zhou Qun-fang,Jiang Gui-bin,&Song Mao-yong.(2007).Determination of alkali-labile phosphoprotein phosphorus from fish plasma using the Tb3+-tiron complex as a fluorescence probe..
MLA	Lv Xue-fei,et al."Determination of alkali-labile phosphoprotein phosphorus from fish plasma using the Tb3+-tiron complex as a fluorescence probe".(2007).

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