| Isolation, purification and characterization of superoxide dismutase from garlic | |
| He, Ning ; Li, Qingbiao ; Li QB(李清彪) ; Sun, Daohua ; Ling, Xueping | |
| 2007-06 | |
| 关键词 | superoxide dismutase garlic purification chromatography |
| 英文摘要 | A simple and cost-effective way to purify superoxide dismutase (SOD) from garlic was proposed. The stepwise isolation and purification procedure consists of phosphate buffer and chloroform-ethanol extraction, acetone precipitation followed by ion exchange chromatography on DEAE column. The specific enzyme activity was 4124 U/mg protein with the recovery rate of 53.3% and concentration factor of 192, respectively. SDS-PAGE analysis showed high consistency of garlic SOD with cattle blood SOD. The enzyme activity of garlic SOD was greatly inhibited by cyanide and hydrogen peroxide while it could be well maintained at pH values ranging from 4 to 11. Only slight decrease of enzyme activity was identified at temperatures lower than 50 degrees C or by treatment with ultrasonic wave for 30 min. The ultraviolet absorbance of garlic SOD was found to be 269 nm. (C) 2007 Elsevier B.V. All rights reserved. |
| 语种 | 英语 |
| 出版者 | ELSEVIER SCIENCE SA |
| 内容类型 | 期刊论文 |
| 源URL | [http://dx.doi.org/doi:10.1016/j.bej.2007.06.005]  |
| 专题 | 化学化工－已发表论文 |
| 推荐引用方式 GB/T 7714 | He, Ning,Li, Qingbiao,Li QB,et al. Isolation, purification and characterization of superoxide dismutase from garlic[J],2007. |
| APA | He, Ning,Li, Qingbiao,李清彪,Sun, Daohua,&Ling, Xueping.(2007).Isolation, purification and characterization of superoxide dismutase from garlic.. |
| MLA | He, Ning,et al."Isolation, purification and characterization of superoxide dismutase from garlic".(2007). |
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