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	A novel, selective, and rapid fluorimetric method for the simultaneous analysis of coproporphyrin and uroporphyrin in urine
	Shindi, Ali Abbas Falih ; Zhou, Pei-Chen ; Zou, Zhe-Xiang ; Li, Yao-Qun ; Li YQ(李耀群)
	2010-02
关键词	Urinary porphyrins Matrix Isopotential Synchronous Fluorescence Spectrometry Porphyria Differential diagnosis
英文摘要	A simple and rapid spectrofluorimetric method is described for the determination of the closely overlapping mixture of coproporphyrin (CP) and uroporphyrin (UP) in urine samples. Matrix Isopotential Synchronous Fluorescence Spectrometry (MISFS) was applied to improve the spectral resolution for the severely overlapped spectra of the urinary porphyrins. First-order derivative technique eliminates the background interference of each component on the other. Using these two techniques together, selectivity was improved, while maintaining a high sensitivity, and time-consuming separation processes and multiple scanning processes were avoided. The limits of detection were 0.15 nmol L-1 and 0.1 nmol L-1 for CP and UP, respectively. The concentrations of CP and UP were determined from the peak amplitudes of the Derivative Matrix Isopotential Synchronous Fluorescence (DMISF) spectra, at their detection points where the interference was suppressed. Porphyrins excretion in urine samples, collected from normal subjects, was studied. A comparison between the new method and the anion-exchange chromatographic method of Martinez and Mills was established using Bland-Altman method and the results indicate that these two methods are in a good agreement with each other. (C) 2010 Elsevier B.V. All rights reserved.; National Natural Science Foundation of China [20575055, 20975084]; National Basic Research Program of China [2007CB935600]
语种	英语
出版者	ELSEVIER SCIENCE BV
内容类型	期刊论文
源URL	[http://dx.doi.org/doi:10.1016/j.aca.2010.01.062]
专题	化学化工－已发表论文
推荐引用方式 GB/T 7714	Shindi, Ali Abbas Falih,Zhou, Pei-Chen,Zou, Zhe-Xiang,et al. A novel, selective, and rapid fluorimetric method for the simultaneous analysis of coproporphyrin and uroporphyrin in urine[J],2010.
APA	Shindi, Ali Abbas Falih,Zhou, Pei-Chen,Zou, Zhe-Xiang,Li, Yao-Qun,&李耀群.(2010).A novel, selective, and rapid fluorimetric method for the simultaneous analysis of coproporphyrin and uroporphyrin in urine..
MLA	Shindi, Ali Abbas Falih,et al."A novel, selective, and rapid fluorimetric method for the simultaneous analysis of coproporphyrin and uroporphyrin in urine".(2010).

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