QQ客服 官方微博 反馈留言

	适于固定化细胞内底物有效扩散系数测定的无反应发生条件; Non-reaction-occurring Condition for Measuring Effective Diffusivity of a Species in Immobilized Cell Matrix
	李薇 ; 陈学云 ; 李清彪
	2000-05
关键词	有效扩散系数 固定化细胞 苯甲酸 葡萄糖 effective diffusivity immobilized cell benzoic acid glucose
英文摘要	[中文文摘]在无反应发生条件下进行组分在固定化细胞内有效扩散系数测定时 ,常常要使细胞失去活性 .通过显微镜形态观察及重量变化测定 ,分析了常用的几种失活细胞的方法对细胞本身的影响 .测定了由各种不同方式得到的无活性固定化细胞内葡萄糖与乙醇的有效扩散系数 ,并与有活性固定化细胞内组分的有效扩散系数进行了比较 .提出了一种方便于使用 ,既可保护底物不被消耗 ,又可以测得更接近实际值的有效扩散系数的无反应发生条件 :苯甲酸处理法。[英文文摘]There exist different methods of inactivating cells which give a non- reaction- occurring condition under which the effective diffusivity of a substrate in the immobilized cell matrix is often measured.Based on micrographic observation of size and shape of the cell and determination of weight change of cells,the effect of the various inactivating methods on the cell itself was examined.The effective diffusivities of glucose and ethanol in the inactivated yeast cell- occupided polyacrylamide gels ob tained f rom the variou s m ethods, w ere determ ined and compared w ith the diffu sivity in the act ive immob ilized cell. So, the method called as " benzoic acid-treating method" has been proposed, which is convienient to work and provides a non-substrate-consuming condition aswell as gives a more reasonab levalue of diffusivity than other methods.
语种	中文
出版者	厦门大学
内容类型	期刊论文
源URL	[http://dspace.xmu.edu.cn/handle/2288/3511]
专题	化学化工－已发表论文
推荐引用方式 GB/T 7714	李薇,陈学云,李清彪. 适于固定化细胞内底物有效扩散系数测定的无反应发生条件, Non-reaction-occurring Condition for Measuring Effective Diffusivity of a Species in Immobilized Cell Matrix[J],2000.
APA	李薇,陈学云,&李清彪.(2000).适于固定化细胞内底物有效扩散系数测定的无反应发生条件..
MLA	李薇,et al."适于固定化细胞内底物有效扩散系数测定的无反应发生条件".(2000).

个性服务

查看访问统计

相关权益政策

暂无数据

收藏/分享

除非特别说明，本系统中所有内容都受版权保护，并保留所有权利。