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Production and Characterization of a Novel Bioflocculant from Bacillus licheniformis
Xiong, Yuyan ; Wang, Yuanpeng ; Yu, Yi ; Li, Qingbiao ; Wang, Haitao ; Chen, Ronghui ; He, Ning ; He N(何宁)
2010-03
英文摘要A bacterium producing an extracellular bioflocculant was isolated from contaminated LB medium and identified as Bacillus licheniformis by 16S rRNA gene sequencing and its biochemical/physiological characteristics. The optimum culture conditions for flocculant production were an initial medium pH of 7.2 and an inoculum size of 4% (vol/vol). The maximum flocculating activity (700 U/ml) was obtained after cultivation at 37 degrees C for 48 h. Chemical analyses of the purified bioflocculant revealed that it was a proteoglycan composed of 89% carbohydrate and 11% protein (wt/wt). The mass ratio of neutral sugar, amino sugar, and uronic acid was measured at 7.9:4:1. Infrared spectrometry further indicated the presence of carboxyl, hydroxyl, and amino groups, typical of heteropolysaccharide. The average mass of the bioflocculant was calculated to be 1.76 x 10(6) Da. Scanning electron microscopy (SEM) images of the bioflocculant showed an irregular structure with netted texture. Its efficient flocculation capabilities suggest potential applications in industry.; National Natural Science Foundation of China [30700020, 40801195]; Xiamen Science and Technology Committee [3502Z20093006]; Ministry of Science and Technology of the People's Republic of China [2009EG111023]
语种英语
出版者AMER SOC MICROBIOLOGY
内容类型期刊论文
源URL[http://dx.doi.org/doi:10.1128/AEM.02558-09]  
专题化学化工-已发表论文
推荐引用方式
GB/T 7714
Xiong, Yuyan,Wang, Yuanpeng,Yu, Yi,et al. Production and Characterization of a Novel Bioflocculant from Bacillus licheniformis[J],2010.
APA Xiong, Yuyan.,Wang, Yuanpeng.,Yu, Yi.,Li, Qingbiao.,Wang, Haitao.,...&何宁.(2010).Production and Characterization of a Novel Bioflocculant from Bacillus licheniformis..
MLA Xiong, Yuyan,et al."Production and Characterization of a Novel Bioflocculant from Bacillus licheniformis".(2010).
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