Production and Characterization of a Novel Bioflocculant from Bacillus licheniformis | |
Xiong, Yuyan ; Wang, Yuanpeng ; Yu, Yi ; Li, Qingbiao ; Wang, Haitao ; Chen, Ronghui ; He, Ning ; He N(何宁) | |
2010-03 | |
英文摘要 | A bacterium producing an extracellular bioflocculant was isolated from contaminated LB medium and identified as Bacillus licheniformis by 16S rRNA gene sequencing and its biochemical/physiological characteristics. The optimum culture conditions for flocculant production were an initial medium pH of 7.2 and an inoculum size of 4% (vol/vol). The maximum flocculating activity (700 U/ml) was obtained after cultivation at 37 degrees C for 48 h. Chemical analyses of the purified bioflocculant revealed that it was a proteoglycan composed of 89% carbohydrate and 11% protein (wt/wt). The mass ratio of neutral sugar, amino sugar, and uronic acid was measured at 7.9:4:1. Infrared spectrometry further indicated the presence of carboxyl, hydroxyl, and amino groups, typical of heteropolysaccharide. The average mass of the bioflocculant was calculated to be 1.76 x 10(6) Da. Scanning electron microscopy (SEM) images of the bioflocculant showed an irregular structure with netted texture. Its efficient flocculation capabilities suggest potential applications in industry.; National Natural Science Foundation of China [30700020, 40801195]; Xiamen Science and Technology Committee [3502Z20093006]; Ministry of Science and Technology of the People's Republic of China [2009EG111023] |
语种 | 英语 |
出版者 | AMER SOC MICROBIOLOGY |
内容类型 | 期刊论文 |
源URL | [http://dx.doi.org/doi:10.1128/AEM.02558-09] ![]() |
专题 | 化学化工-已发表论文 |
推荐引用方式 GB/T 7714 | Xiong, Yuyan,Wang, Yuanpeng,Yu, Yi,et al. Production and Characterization of a Novel Bioflocculant from Bacillus licheniformis[J],2010. |
APA | Xiong, Yuyan.,Wang, Yuanpeng.,Yu, Yi.,Li, Qingbiao.,Wang, Haitao.,...&何宁.(2010).Production and Characterization of a Novel Bioflocculant from Bacillus licheniformis.. |
MLA | Xiong, Yuyan,et al."Production and Characterization of a Novel Bioflocculant from Bacillus licheniformis".(2010). |
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