Quantum Dot-Based Immunochromatographic Fluorescent Biosensor for Biomonitoring Trichloropyridinol, a Biomarker of Exposure to Chlorpyrifos | |
Zou, Zhexiang ; Du, Dan(Pacific NW Natl Lab, Richland) ; Wang, Jun(Pacific NW Natl Lab, Richland) ; Smith, Jordan N.(Pacific NW Natl Lab, Richland) ; Timchalk, Charles(Pacific NW Natl Lab, Richland) ; Li, Yaoqun ; Li YQ(李耀群) ; Lin, Yuehe(Pacific NW Natl Lab, Richland) | |
2010-05 | |
英文摘要 | A novel and portable fluorescent sensor that integrates an immunochromatographic test strip assay (ITSA) with a quantum dot (QD) label and a test strip reader was described in this study for simple, rapid, and sensitive biomonitoring of an organophosphorus pesticide metabolite. The principle of this sensor is based on a competitive immunoreaction that was performed on an immunochromatographic test strip, where analytes compete with competitors (QD-conjugated analogs) to bind to antibodies on a test zone. Captured QDs serve as signal vehicles for fluorescent readout. In this work, 3,5,6-trichloropyridinol (TCP) is used as a model analyte to demonstrate the performance of the immunosensor. QD-TCP conjugates were synthesized and characterized with X-ray photoelectron spectroscopy (XPS) and fluorescence spectroscopy. Some parameters (e.g., the amount of QD-modified TCP and immunoreaction time) that govern sensitivity and reproducibility of ITSA were optimized. Under optimal conditions, the sensor has a wide dynamic range and is capable of detecting a minimum 1.0 ng/mL TCP standard analyte in 15 min. The sensor has been successfully applied for detection of TCP spiked in rat plasma with average recovery of 102.0%. Results demonstrate that this sensor provides a rapid, clinically accurate, and quantitative tool for TCP detection and shows great promise for in-field and point-of-care (POC) quantitative testing and screening for metabolite biomarkers, e.g., TCP, for humans exposed to pesticides.; Centers for Disease Control/National Institute for Occupational Safety and Health [R01 OH008173-01]; NIH through the National Institute of Neurological Disorders and Stroke [U01 NS058161-01]; China Scholarship Council ; PNNL |
语种 | 英语 |
出版者 | AMER CHEMICAL SOC |
内容类型 | 期刊论文 |
源URL | [http://dx.doi.org/doi:10.1021/ac100260m] ![]() |
专题 | 化学化工-已发表论文 |
推荐引用方式 GB/T 7714 | Zou, Zhexiang,Du, Dan,Wang, Jun,et al. Quantum Dot-Based Immunochromatographic Fluorescent Biosensor for Biomonitoring Trichloropyridinol, a Biomarker of Exposure to Chlorpyrifos[J],2010. |
APA | Zou, Zhexiang.,Du, Dan.,Wang, Jun.,Smith, Jordan N..,Timchalk, Charles.,...&Lin, Yuehe.(2010).Quantum Dot-Based Immunochromatographic Fluorescent Biosensor for Biomonitoring Trichloropyridinol, a Biomarker of Exposure to Chlorpyrifos.. |
MLA | Zou, Zhexiang,et al."Quantum Dot-Based Immunochromatographic Fluorescent Biosensor for Biomonitoring Trichloropyridinol, a Biomarker of Exposure to Chlorpyrifos".(2010). |
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