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	Metabonomics Research of Diabetes Mellitus and Diabetic Nephropathy Based on UPLC-oaTOF-MS System
	Zhang, Jie ; Zhang J(张洁) ; Yan, Li-Juan ; Yan LJ(严丽娟) ; Lin, Lin ; Lin L(林琳) ; Chen, Wen-Gui ; Chen WG(陈文贵) ; Song, Xiu-Yu ; Song XY(宋秀宇) ; Yan, Xiao-Mei ; Yan XM(颜晓梅) ; Hang, Wei ; Hang W(杭纬) ; Huang, Ben-Li ; Huang BL(黄本立)
	2008
关键词	Diabete mellitus Diabetic nephropathy Metabonomics Ultra performance liquid chromatography Time-of-flight mass spectrometer
英文摘要	Ultra performance liquid chromatography (UPLC) Coupled with orthogonal acceleration time-of-flight( (oaTOF) mass spectrometry is becoming an effective and high-throughput analytical technique in metabonomics research. In this paper, an UPLC-oaTOF-Ms system was employed to investigate the serum profiles of 40 diabete mellitus(DM)/diabetic nephropathy(DN) patients and 25 healthy volunteers in an effort to find potential biomarkers. The UPLC system produced information-rich chromatograms with typical measured peak widths of 4 s, and generated peak capacities of 225 in 15 min. After the UPLC-oaTOF-MS analysis, principal component analysis (PCA) was used for group differentiation and marker selection. In the projection of positive mode, DM/DN patients and the controls were obviously divided into three groups. Furthermore, two potential markers were identified as phytosphingosine and sphinganine, respectively. Phytosphingosine and sphinganine with decreased concentrations in the patients, were closely related to the sphingolipid metabolism.
语种	英语
出版者	HIGHER EDUCATION PRESS
内容类型	期刊论文
源URL	[http://dx.doi.org/doi:CNKI:SUN:GDXH.0.2008-11-011]
专题	化学化工－已发表论文
推荐引用方式 GB/T 7714	Zhang, Jie,Zhang J,Yan, Li-Juan,et al. Metabonomics Research of Diabetes Mellitus and Diabetic Nephropathy Based on UPLC-oaTOF-MS System[J],2008.
APA	Zhang, Jie.,张洁.,Yan, Li-Juan.,严丽娟.,Lin, Lin.,...&黄本立.(2008).Metabonomics Research of Diabetes Mellitus and Diabetic Nephropathy Based on UPLC-oaTOF-MS System..
MLA	Zhang, Jie,et al."Metabonomics Research of Diabetes Mellitus and Diabetic Nephropathy Based on UPLC-oaTOF-MS System".(2008).

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