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	基于AFLP分子标记探讨福建近海竹荚鱼群体遗传多样性; New Evidence to the Genetic Diversity of Trachurus japonicus in the Coastal Waters of Fujian Province Based on AFLP Makers
	张丽艳 ; 牛素芳 ; 张曼 ; 王军
	2014-01-28
关键词	竹荚鱼 遗传分化 遗传多样性 扩增片段长度多态性(AFLP) Trachurus japonicus genetic differentiation genetic diversity amplified fragment length polymorphism(AFLP)
英文摘要	为研究福建近海竹荚鱼群体的遗传分化,对竹荚鱼闽东(30尾)和闽南(30尾)群体进行了扩增片段长度多态性(AflP)分析,8对选择性引物在2个群体60个个体中,共扩增出433条条带,其中多态位点286个.闽东和闽南群体的多态位点比例分别为63.28%、61.89%,nEI遗传多样性指数分别为0.171 8、0.172 2,SHAnnOn多样性指数分别为0.267 3、0.267 3.与其他鱼类对比显示,福建近海竹荚鱼群体的遗传多样性水平较高,说明其种质资源尚未遭到明显破坏;遗传分化系数(gST)和分子生物学方差分析(AMOVA)均显示竹荚鱼的遗传变异主要来源于群体内,而群体间无明显的遗传分化.基因流(nM)显示2个群体间基因交流频繁,无明显独立的遗传结构.群体的位点差异数分布和显性基因型频率分布显示2个群体有相似的群体遗传结构.结果表明,竹荚鱼闽东和闽南群体间无明显的遗传差异,可将福建近海的竹荚鱼划归同一个管理保护单元.近期扩张、较强的扩散能力和洋流可能是造成福建近海竹荚鱼群体间遗传同质性较高的原因.; Given Trachurus japonicus resources declining,amplified fragment length polymorphism(AFLP)was employed to analyze the genetic diversity and variation of two populations of T.japonicus from Eastern and Southern Fujian coastal water in this study.AFLP bands were scored for presence(1)or absence(0)and transformed into 0/1binary character matrix.AMOVA and mismatch distribution analysis were performed in Arlequin,Nei genetic diversity and Shannon genetic diversity index were conducted in POPGEN,genetic distance between individuals were computed in Phyltools and the UPGMA tree was generated in MEGA.A total of 433loci ranging in size from 100bp to 1 000bp were detected from 60individuals using eight primer combinations,of which 286 were polymorphic.The proportion of polymorphic loci,the Nei genetic diversity and Shannon genetic diversity index of these two populations were 63.28%and 61.89%,0.171 8and 0.172 2,0.267 3and 0.267 3,respectively,revealing no significant difference(p>0.05)between the two populations.By comparison with that of other fishes,that the genetic diversity of T.japonicus in the coastal waters of Fujian province is above the middle level and the population genetic structure of T.japonicus had not been destroyed.Gst value,Shannon genetic diversity index and AMOVA analysis indicated that the genetic variation mainly came from individuals within populations.Nm showed that there was frequent gene flow between the two populations.The NJ tree based on genetic distance between individuals exhibited no correlation to geographic distribution.Mismatch distribution analysis and dominant gene frequency revealed that these two populations have the similar genetic structure.Based on the facts above,the results of AFLP analysis indicated that there is no significant genetic differentiation between two populations,and the recent expansion,annual migrations and larval drift in the ocean currents could be the reasons for the high homogeneity of genetic structure between two populations.The knowledge on genetic diversity and variation will be crucial to establish appropriate fishery management stocks for the species.; 福建省科技计划项目(2013N0005;2007I0005)
语种	zh_CN
内容类型	期刊论文
源URL	[http://dspace.xmu.edu.cn/handle/2288/104721]
专题	海洋环境－已发表论文
推荐引用方式 GB/T 7714	张丽艳,牛素芳,张曼,等. 基于AFLP分子标记探讨福建近海竹荚鱼群体遗传多样性, New Evidence to the Genetic Diversity of Trachurus japonicus in the Coastal Waters of Fujian Province Based on AFLP Makers[J],2014.
APA	张丽艳,牛素芳,张曼,&王军.(2014).基于AFLP分子标记探讨福建近海竹荚鱼群体遗传多样性..
MLA	张丽艳,et al."基于AFLP分子标记探讨福建近海竹荚鱼群体遗传多样性".(2014).

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