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	海马齿根际降解可溶性蛋白质的研究; Study on Rhizodegradation of Soluble Protein by Sesuvium portulacastrum Linn.
	杨芳 ; 李凯 ; 黄凌风 ; 朱小明
	2014-01-28
关键词	海马齿 亮氨酸氨基肽酶活性 牛血清白蛋白 根际微生物 根际降解 Sesuvium portulacastrum Linn. leucine amino peptidase activity(LAPA) bovine serum albumin(BSA) rhizosphere microbe rhizodegradation
英文摘要	采用水培法研究了海马齿(SESuVIuM POrTulACASTruM lInn.)与其根际微生物对可溶性蛋白质的联合降解作用.分别设置抑菌和不抑菌的植物组、细菌组和对照组并连续添加蛋白质,测定了降解过程中蛋白质的浓度、细菌密度和亮氨酸氨基肽酶活性的变化.结果显示,在不抑菌实验中,植物组的蛋白质浓度下降最快.36H后,植物组、细菌组和对照组的降解率分别是97%、41%和26%.蛋白质的降解过程伴随着细菌数量的增加和亮氨酸氨基肽酶活性的升高,植物组的亮氨酸氨基肽酶活性始终高于细菌组.24H后,植物组的亮氨酸氨基肽酶活性达到最高值0.75μMOl/(l·H),此后酶活性下降至0.22μMOl/(l·H).细菌组的亮氨酸氨基肽酶活性最高值出现在60H,达到0.43μMOl/(l·H),至结束时降低为0.说明海马齿与根际细菌联合作用下,胞外酶活性升高,蛋白质降解效率最快.在抑菌实验中,抗生素有效抑制了细菌的生长,3个处理组的蛋白质变化趋势基本一致,实验过程中检测不到亮氨酸氨基肽酶,说明单纯的海马齿根系不分泌该胞外酶.研究表明,在蛋白质的根际降解过程中,根际细菌是亮氨酸氨基肽酶的分泌者和蛋白质降解的主要执行者,但根际细菌对蛋白质的高效降解必须以海马齿根系为依托.本研究结果为更好地利用海马齿生态浮床修复海水环境中的有机氮提供了理论依据.; Phytoremediation of soluble protein by marine halophyte Sesuvium portulacastrum which has been supposed to use in ecofloating bed systems was studied through a hydroponic culture approach.The plant group,the bacteria group and the control group were treated with and without antibiotic addition.The results showed that,without antibiotic addition,each time after bovine serum albumin(BSA)was added,the BSA concentration of the plant group dropped fastest.After 36hincubation,the degradation rate of the plant group,the bacteria group and the control group was 97%,41%and 26%respectively,i.e.the degradation rate of the plant group was significantly higher than the bacteria group.Protein degradation associated with the increasing of both leucine amino peptidase activity(LAPA)and bacterial density,and LAPA of the plant group was always higher than that of the bacteria group.Soon after the experiment started,LAPA of the plant group increased rapidly and reached its maximum value of 0.75μmol/(L·h)at 24h.Then LAPA started to decline to 0.22μmol/(L·h)till the experiment ended.The maximum LAPA value of the bacteria group was 0.43μmol/(L·h)at 60h,and till the experiment ended,the LAPA was 0μmol/(L·h).With antibiotic addition,the bacterial density of both plant group and bacteria group showed downtrend,and it showed that the antibiotic inhibited the growth of bacteria effectively.The degradation rates of 3groups were almost the same,and LAPA was not detected in the procession.It was suggested that in the procession of rhizodegradation of BSA by S.portulacastrum,the extracellular enzyme was produced by rhizosphere bacteria,and the rhizoshpere microbe was the main performer of BSA degradation.Besides,the high degradation rates of rhizosphere bacteria relied on the root of S.portulacastrum.The results provide meaningful insights into the interrelationship between microbes and plant as well as the practical significance of soluble protein rhizodegradation in seawater environments by eco-floating bed system.; 国家海洋公益性行业科研专项(201205009); 深圳市科技研发资金(JCYJ20120830153030587)
语种	zh_CN
内容类型	期刊论文
源URL	[http://dspace.xmu.edu.cn/handle/2288/104718]
专题	海洋环境－已发表论文
推荐引用方式 GB/T 7714	杨芳,李凯,黄凌风,等. 海马齿根际降解可溶性蛋白质的研究, Study on Rhizodegradation of Soluble Protein by Sesuvium portulacastrum Linn.[J],2014.
APA	杨芳,李凯,黄凌风,&朱小明.(2014).海马齿根际降解可溶性蛋白质的研究..
MLA	杨芳,et al."海马齿根际降解可溶性蛋白质的研究".(2014).

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