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	急性氨氮胁迫对于草鱼sod和hsp90基因表达及鳃部结构的影响(英文); THE EFFECT of HIGH AMMONIA CONCENTRATION ON GILL STRUCTURE ALTERNATION AND EXPRESSION of SOD AND HSP90 GENES IN GRASS CARP,CTENOPHARYNGODON IDELLA
	周鑫 ; 董云伟 ; 王芳 ; 董双林
	2013-03-15
关键词	草鱼 氨氮暴露 24h半致死浓度 组织损伤 sod hsp90 Grass carp Ammonia exposure 24h LC 50 Histological damage sod hsp90
英文摘要	实验通过测定草鱼的24H半致死浓度,鳃的细胞结构以及SOd和HSP90的表达模式研究了草鱼在组织学和分子生物学水平上对高浓度氨氮暴露的响应。经过半致死实验确定的氨氮24H lC50为243 Mg/l试验中草鱼被置于5个浓度的处理组中(50、72、104、151、220 Mg/l),之后取鳃组织进行组织切片分析,取肝脏、肠和鳃来测定SOd和HSP90的表达情况。经过高浓度的氨氮暴露处理,鳃组织的细胞排列和结构产生了明显的变化,并且SOd和HSP90的表达受到了显著的上调。这些结果表明,高浓度的氨氮能够损害鳃部的细胞结构并且诱导应激蛋白的表达。这个结果同样显示出,SOd和HSP90可以作为评估草鱼氨氮暴露水平的良好指标。; Histological and molecular responses of grass carp Ctenopharyngodon idella to high concentration ammonia exposure were studied in this study by determining the lethal concentration,gill cellular structure,and expression patterns of sod and hsp90 genes.Grass carps were treated at five ammonia concentrations(50,72,104,151 and 220 mg/L) for 24h,and then the gill tissues were collected for histological analysis.In addition,three tissues(gill,intestine and liver) were collected to measure the expression of sod and hsp90 genes.After exposure to high concentration of ammonia,the arrangement and structures of gill cells changed dramatically.Edema and fusion of secondary lamellae were observed,and the edge of cell and nucleus could not be clearly defined.Meanwhile,the two genes(sod and hsp90) in different tissues were unregulated significantly,indicating that high concentration of ammonia could impair the cellular structures and induce the expression of stress proteins.These results also suggested that sod and hsp90 were suitable biomarkers for ammonia exposure assessment in grass carp.; FoundsoftheNationalProgramonKeyBasicResearchProject(2009CB118706)
语种	zh_CN
内容类型	期刊论文
源URL	[http://dspace.xmu.edu.cn/handle/2288/104597]
专题	海洋环境－已发表论文
推荐引用方式 GB/T 7714	周鑫,董云伟,王芳,等. 急性氨氮胁迫对于草鱼sod和hsp90基因表达及鳃部结构的影响(英文), THE EFFECT of HIGH AMMONIA CONCENTRATION ON GILL STRUCTURE ALTERNATION AND EXPRESSION of SOD AND HSP90 GENES IN GRASS CARP,CTENOPHARYNGODON IDELLA[J],2013.
APA	周鑫,董云伟,王芳,&董双林.(2013).急性氨氮胁迫对于草鱼sod和hsp90基因表达及鳃部结构的影响(英文)..
MLA	周鑫,et al."急性氨氮胁迫对于草鱼sod和hsp90基因表达及鳃部结构的影响(英文)".(2013).

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