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	安氏伪镖水蚤精氨酸激酶cDNA的克隆及镍胁迫下的表达特性; cDNA Cloning of Arginine Kinase(AK) from Pseudodiaptomus annandalei and the Expression Pattern of AK Under Nickel Stress
	蒋洁兰 ; 王桂忠 ; 毛明光 ; 李少菁
	2013-07-28
关键词	安氏伪镖水蚤 精氨酸激酶 镍胁迫 表达特性 Pseudodiaptomus annandalei arginine kinase nickel stress expression characterization
英文摘要	为探讨镍胁迫对桡足类影响的分子机制,以安氏伪镖水蚤(PSEudOdIAPTOMuS AnnAndAlEI)为研究对象,采用Cd-nA末端快速扩增技术(rACE)方法获得了安氏伪镖水蚤精氨酸激酶(ArgInInE kInASE,Ak)CdnA全序列,全长1 180bP,开放阅读框1 068bP,编码355个氨基酸残基,预测蛋白质分子质量为39.43ku.blAST分析结果显示,安氏伪镖水蚤AkCdnA与罗氏沼虾(MACrObrACHIuM rOSEnbErgII)、多齿新米虾(nEOCArIdInA dEnTICulATA)和光滑双脐螺(bI-OMPHAlArIA glAbrATA)Ak基因具有较高的同源性(77%~82%).荧光定量PCr结果显示:低质量浓度镍(10μg/l)对Ak转录水平呈现峰值效应,高质量浓度(40和500μg/l)镍则对Ak呈现抑制效应,表明Ak与安氏伪镖水蚤响应重金属胁迫密切相关.本研究为揭示镍胁迫对桡足类的影响机制提供理论依据.; To study the molecular response mechanisms of copepod to nickel stress,the full-length cDNA of arginine kinase gene from the copepod Pseudodiaptomus annandalei was cloned using the strategy of rapid amplification of cDNA ends(RACE).The arginine kinase cDNA(Pa-AK) was 1 180 bp in length including an open reading frame of 1 068 bp continuously encoded 355 amino acid residues,and the GenBank accession number was JX624126.The molecular weight of the predicted protein was 39.43 ku.Pa-AK showed high homology(77%-82%) with AKs from Macrobrachium rosenbergii,Neocaridina denticulate and Biomphalaria glabrata.Further,the transcripts of Pa-AK under nickel stress were examined using the real-time quantitative PCR technique.The results show that:low concentration of nickel(10 μg/L) first induced transcripts of Pa-AK,at 12 h,the transcript level started to decreased;high concentrations(40 and 500 μg/L) of nickel inhibited transcripts of Pa-AK.The results showed that Pa-AK is closely related to the response to heavy metal stress in Pseudodiaptomus annandalei.The successfully cloning of AK cDNA in Pseudodiaptomus annandalei and the study of expression characterization of Pa-AK under nickel stress will facilitate the study of the molecular mechanisms of nickel stress on copepod from proteomics aspects.; 国家自然科学基金项目(41276132)
语种	zh_CN
内容类型	期刊论文
源URL	[http://dspace.xmu.edu.cn/handle/2288/104559]
专题	海洋环境－已发表论文
推荐引用方式 GB/T 7714	蒋洁兰,王桂忠,毛明光,等. 安氏伪镖水蚤精氨酸激酶cDNA的克隆及镍胁迫下的表达特性, cDNA Cloning of Arginine Kinase(AK) from Pseudodiaptomus annandalei and the Expression Pattern of AK Under Nickel Stress[J],2013.
APA	蒋洁兰,王桂忠,毛明光,&李少菁.(2013).安氏伪镖水蚤精氨酸激酶cDNA的克隆及镍胁迫下的表达特性..
MLA	蒋洁兰,et al."安氏伪镖水蚤精氨酸激酶cDNA的克隆及镍胁迫下的表达特性".(2013).

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