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	TATPTD-Tachyplesin融合基因的重叠延伸PCR法合成; The Artificial Synthesis of TAT PTD and Tachyplesin Fusion Gene by Overlap Extension PCR
	邱道寿 ; 刘晓津 ; 王军 ; 苏永全
	2013-02-01
关键词	PTD TAT 鲎素 重叠延伸PCR TAT protein transduction domain Tachyplesin Overlap extension PCR
英文摘要	为探讨HIV-TAT蛋白转导域(PrOTEIn TrAnSduCTIOn dOMAIn,PTd)对中国鲎抗菌肽TACHyPlESIn的协同作用,试验以经毕赤酵母密码子优化后的TACHyPlESIn成熟肽(54 AA)加TAT PTd(11 AA)的CdnA序列为参考模板,设计了6条单链寡核苷酸片段,首尾引物的5'端分别引入ECOr I和XbA I酶切位点,通过重叠延伸PCr方法成功合成了TAT PTd+TACHyPlESIn序列,序列全长219 bP,为其后续功能与协同作用研究奠定了基础。; In order to study the coefficient of the TAT PTD(the protein transduction domain in HIV-1 transactivator of transcription protein) to the antibacterial peptide tachyplesin from Tachpleus tridentatus,6 sequence of oligonucleotides with EcoR Iand Xba I in the ends of 5′-end of the first and last primers respectively were designed,based on the TAT PTD and tachyplesin mature peptide cDNA template,which was optimized according to the Pichia pastoris preferred codon.A optimized TAT PTD and tachyplesin fusion gene with 219 base pairs was artificially synthesized by overlap extension PCR,which laid a preliminary foundation for the following functional and coefficient studies.; 广东省自然科学基金(06025389); 福建省博士后项目
语种	zh_CN
内容类型	期刊论文
源URL	[http://dspace.xmu.edu.cn/handle/2288/104558]
专题	海洋环境－已发表论文
推荐引用方式 GB/T 7714	邱道寿,刘晓津,王军,等. TATPTD-Tachyplesin融合基因的重叠延伸PCR法合成, The Artificial Synthesis of TAT PTD and Tachyplesin Fusion Gene by Overlap Extension PCR[J],2013.
APA	邱道寿,刘晓津,王军,&苏永全.(2013).TATPTD-Tachyplesin融合基因的重叠延伸PCR法合成..
MLA	邱道寿,et al."TATPTD-Tachyplesin融合基因的重叠延伸PCR法合成".(2013).

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