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	LC-MS-MS测定河口及近岸海水中的氟喹诺酮类药物; Measurement of Fluoroquinolone Antibiotics in Estuarine and Coastal Seawater by Liquid Chromatography-Tandem Mass Spectrometry
	杨琳 ; 黄东仁 ; 温裕云
	2011
关键词	氟喹诺酮 河口及近岸海水 固相萃取 液相色谱 串级质谱 fluoroquinolone antibiotics estuarine and coastal seawater solid phase extraction liquid chromatography tandem mass spectrometry
英文摘要	建立了河口及近岸海水中氟喹诺酮类药物残留的固相萃取富集-高效液相色谱-串级质谱测定方法.以WATErS Hlb固相萃取(SPE)小柱富集水样中的氟喹诺酮类药物,用Xdb-C18柱、乙腈、甲酸(体积分数为0.2%)水流动相进行分离,ESI正离子源、三重串级质谱及多反应监测模式(MrM)进行目标物的定性、定量测定.优化实验条件下,方法的检出限(3σ)为0.002~0.010μg/l,定量限(10σ)为0.010~0.050μg/l,样品加标回收率为73.3%(奥比沙星)~119.7%(氧氟沙星),相对标准偏差(rSd)为1.3%~5.8%(2.5μg/l,n=6).; An accurate method was developed for measurement of fluoroquinolone antibiotics in estuarine and coastal seawater by solid phase extraction(SPE) and liquid chromatography-tandem mass spectrometry.The fluoroquinolones were enriched by Waters HLB SPE column,eluted and concentrated before separating with XDB-C18 column with mobile phase of acetonitrile and water containing 0.2 % formic acid.The analytes were then detected with tandem mass spectrometer using positive ESI at MRM mode.Under the optimized operating conditions,relative standard deviation(RSD) for developed method were 1.3 %--5.8 %(2.5 μg/L,n=6),standard added recoveries were 73.3 %(orbifloxacin) --119.7 %(ofloxacin),limits of detection(3σ) were 0.002--0.010 μg/L,limits of quantification(10σ)were 0.010--0.050 μg/L.; 海洋公益性行业科研专项(200705029)
语种	zh_CN
内容类型	期刊论文
源URL	[http://dspace.xmu.edu.cn/handle/2288/104322]
专题	海洋环境－已发表论文
推荐引用方式 GB/T 7714	杨琳,黄东仁,温裕云. LC-MS-MS测定河口及近岸海水中的氟喹诺酮类药物, Measurement of Fluoroquinolone Antibiotics in Estuarine and Coastal Seawater by Liquid Chromatography-Tandem Mass Spectrometry[J],2011.
APA	杨琳,黄东仁,&温裕云.(2011).LC-MS-MS测定河口及近岸海水中的氟喹诺酮类药物..
MLA	杨琳,et al."LC-MS-MS测定河口及近岸海水中的氟喹诺酮类药物".(2011).

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