Polymorphic microsatellite markers in the false kelpfish Sebastiscus marmoratus: isolation, characterization, and cross-species amplification

CORC > 厦门大学 > 海洋环境－已发表论文

	Polymorphic microsatellite markers in the false kelpfish Sebastiscus marmoratus: isolation, characterization, and cross-species amplification
	Liu, H. B. ; Liu, S. F. ; Ye, J. B. ; Yuan, Y. J. ; Ding, S. X. ; Zhuang, Z. M. ; Ding SX(丁少雄)
刊名	http://dx.doi.org/10.4238/2014.January.10.4
	2014
关键词	ROCKFISH
英文摘要	Public Science and Technology Research Founds Projects of Ocean [201205024]; National Natural Science Foundation of China [31061160187]; A (GT/CA)(13)-microsatellite-enriched genomic library of the false kelpfish Sebastiscus marmoratus was constructed, and 20 polymorphic microsatellite loci were isolated and characterized. The polymorphisms were investigated in 48 wild individuals from a single population collected from the northern Yellow Sea. The numbers of alleles per locus varied from 4-22 with an average of 9. The observed and expected heterozygosities of each locus ranged from 0.196-0.958 and from 0.487-0.942, with an average of 0.693 and 0.765, respectively. One locus significantly deviated from Hardy-Weinberg equilibrium, and one pair of loci was in linkage disequilibrium determined by Bonferroni's correction. Cross-species amplification was also conducted in the related species Inimicus japonicus, collected from East China Sea. The result showed that six loci could be amplified from I. japonicus DNAs. These polymorphic markers would be useful for assessment of genetic variation and population structure of scorpionfish.
语种	英语
出版者	FUNPEC-EDITORA
内容类型	期刊论文
源URL	[http://dspace.xmu.edu.cn/handle/2288/88196]
专题	海洋环境－已发表论文
推荐引用方式 GB/T 7714	Liu, H. B.,Liu, S. F.,Ye, J. B.,et al. Polymorphic microsatellite markers in the false kelpfish Sebastiscus marmoratus: isolation, characterization, and cross-species amplification[J]. http://dx.doi.org/10.4238/2014.January.10.4,2014.
APA	Liu, H. B..,Liu, S. F..,Ye, J. B..,Yuan, Y. J..,Ding, S. X..,...&丁少雄.(2014).Polymorphic microsatellite markers in the false kelpfish Sebastiscus marmoratus: isolation, characterization, and cross-species amplification.http://dx.doi.org/10.4238/2014.January.10.4.
MLA	Liu, H. B.,et al."Polymorphic microsatellite markers in the false kelpfish Sebastiscus marmoratus: isolation, characterization, and cross-species amplification".http://dx.doi.org/10.4238/2014.January.10.4 (2014).