16S rRNA is a better choice than COI for DNA barcoding hydrozoans in the coastal waters of China

CORC > 厦门大学 > 海洋环境－已发表论文

	16S rRNA is a better choice than COI for DNA barcoding hydrozoans in the coastal waters of China
	Zheng Lianming ; He Jinru ; Lin Yuanshao ; Cao Wenqing ; Zhang Wenjing ; Zheng LM(郑连明) ; Lin YS(林元烧) ; Cao WQ(曹文清) ; Zhang WJ(张文静)
刊名	http://dx.doi.org/10.1007/s13131-014-0415-8
	2014
关键词	EUROPEAN ATHECATE HYDROIDS REVEALS CRYPTIC DIVERSITY MOLECULAR PHYLOGENETICS CNIDARIA HYDROZOA MEDUSAE HYDROZOA LIFE-CYCLE SEQUENCE EVOLUTION RDNA DATA N. SP MITOCHONDRIAL
英文摘要	National Natural Science Foundation of China [41006078]; Fundamental Research Funds for the Central Universities [2010121037]; Public Science and Technology Research Funds Projects of Ocean [201005012-3, 201005015-5]; Natural Science Foundation of Fujian Province of China [2011J05116]; Identification of hydrozoan species is challenging, even for taxonomic experts, due to the scarcity of distinct morphological characters and phenotypic plasticity DNA barcoding provides an efficient method for species identification, however, the choice between mitochondrial cytochrome c oxidase subunit I (COI) and large subunit ribosomal RNA gene (16S) as a standard barcode for hydrozoans is subject to debate. Herein, we directly compared the barcode potential of COI and 16S in hydrozoans using 339 sequences from 47 pelagic hydrozoan species. Analysis of Kimura 2-parameter genetic distances (K2P) documented the mean intraspecific/interspecific variation for COI and 16S to be 0.004/0.204 and 0.003/0.223, respectively. An obvious "barcoding gap" was detected for all species in both markers and all individuals of a species clustered together in both the COI and 16S trees. These results suggested that the species within the studied taxa can be efficiently and accurately identified by COI and 16S. Furthermore, our results confirmed that 16S was a better phylogenetic marker for hydrozoans at the genus level, and in some cases at the family level. Considering the resolution and effectiveness for barcoding and phylogenetic analyses of Hydrozoa, we strongly recommend 16S as the standard barcode for hydrozoans.
语种	英语
出版者	SPRINGER
内容类型	期刊论文
源URL	[http://dspace.xmu.edu.cn/handle/2288/88150]
专题	海洋环境－已发表论文
推荐引用方式 GB/T 7714	Zheng Lianming,He Jinru,Lin Yuanshao,et al. 16S rRNA is a better choice than COI for DNA barcoding hydrozoans in the coastal waters of China[J]. http://dx.doi.org/10.1007/s13131-014-0415-8,2014.
APA	Zheng Lianming.,He Jinru.,Lin Yuanshao.,Cao Wenqing.,Zhang Wenjing.,...&张文静.(2014).16S rRNA is a better choice than COI for DNA barcoding hydrozoans in the coastal waters of China.http://dx.doi.org/10.1007/s13131-014-0415-8.
MLA	Zheng Lianming,et al."16S rRNA is a better choice than COI for DNA barcoding hydrozoans in the coastal waters of China".http://dx.doi.org/10.1007/s13131-014-0415-8 (2014).