Molecular cloning, expression profiles and subcellular localization of cyclin B in ovary of the mud crab, Scylla paramamosain

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	Molecular cloning, expression profiles and subcellular localization of cyclin B in ovary of the mud crab, Scylla paramamosain
	Li, Wen-Xing ; Huang, Hui-Yang ; Huang, Jing-Ru ; Yu, Jin-Jin ; Ma, Jun ; Ye, Hai-Hui ; Huang HY(黄辉洋) ; Ye HH(叶海辉)
刊名	http://dx.doi.org/10.1007/s13258-013-0077-5
	2013
关键词	MESSENGER-RNA POLYADENYLATION CELL-CYCLE DEPENDENT KINASES OOCYTE MATURATION MOUSE OOCYTES MEIOSIS-I PRE-MPF A-TYPE XENOPUS IDENTIFICATION
英文摘要	National Natural Science Foundation of China [40406030, 41076081]; A full-length cDNA of cyclin B was isolated from ovary of the mud crab (Scylla paramamosain) in this study. This transcript encodes a polypeptide of 401 amino acids, which is highly homologous to cyclin B protein family. Reverse transcription-PCR (RT-PCR) showed that cyclin B mRNA was expressed at highest levels in ovary of the mud crab. During the ovarian maturation process, real-time RT-PCR revealed that the abundance of cyclin B mRNA increased from the second stage (early-developing stage) to the fourth stage (nearly-ripe stage) and reached the peak level at the fifth stage (ripe stage). This result indicates the identified cyclin B gene might be related to the cell proliferation in ovary, both mitotically and meiotically. Immunohistochemistry showed that cyclin B protein was localized in the cytoplasm of prophase oocytes at the second stage while enriched in the nuclei of pro-metaphase oocytes at the fourth stage. It suggests the tested cyclin B protein might play different roles in ovary at the two stages.
语种	英语
出版者	SPRINGER
内容类型	期刊论文
源URL	[http://dspace.xmu.edu.cn/handle/2288/88055]
专题	海洋环境－已发表论文
推荐引用方式 GB/T 7714	Li, Wen-Xing,Huang, Hui-Yang,Huang, Jing-Ru,et al. Molecular cloning, expression profiles and subcellular localization of cyclin B in ovary of the mud crab, Scylla paramamosain[J]. http://dx.doi.org/10.1007/s13258-013-0077-5,2013.
APA	Li, Wen-Xing.,Huang, Hui-Yang.,Huang, Jing-Ru.,Yu, Jin-Jin.,Ma, Jun.,...&叶海辉.(2013).Molecular cloning, expression profiles and subcellular localization of cyclin B in ovary of the mud crab, Scylla paramamosain.http://dx.doi.org/10.1007/s13258-013-0077-5.
MLA	Li, Wen-Xing,et al."Molecular cloning, expression profiles and subcellular localization of cyclin B in ovary of the mud crab, Scylla paramamosain".http://dx.doi.org/10.1007/s13258-013-0077-5 (2013).