A phosphorescent silver(I)-gold (I) cluster complex that specifically lights up the nucleolus of living cells with FLIM imaging

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	A phosphorescent silver(I)-gold (I) cluster complex that specifically lights up the nucleolus of living cells with FLIM imaging
	Chen, Min ; Lei, Zhen ; Feng, Wei ; Li, Chunyan ; Wang, Quan-Ming ; Li, Fuyou ; Chen M(陈敏) ; Wang QM(王泉明) ; Li CY(李春园)
刊名	http://dx.doi.org/10.1016/j.biomaterials.2013.02.032
	2013
关键词	ORGANIZER REGIONS OPTICAL PROBES LIVE CELLS MECHANISM IRIDIUM(III) MICROSCOPY PROTEINS CYCLE
英文摘要	China National Funds for Distinguished Young Scientists from the NSFC [20825101, 21231004]; SSTC [11XD1400200, 10431903100]; State Key Basic Research Program of China [2012CB932403]; SLADP [B108]; The phosphorescent silver(I)-gold(I) cluster complex [CAu6Ag2(dppy)(6)](BF4)(4) (N1) selectively stains the nucleolus, with a much lower uptake in the nucleus and cytoplasm, and exhibits excellent photostability. This Ag-Au cluster, which has a photoluminescent lifetime of microseconds, is particularly attractive as a probe in applications of time-gated microscopy. Investigation of the pathway of cellular entry indicated that N1 permeates the outer membrane and nuclear membrane of living cells through an energy-dependent and non-endocytic route within 10 mm. High concentrations of N1 in the nucleolus have been quantified by inductively coupled plasma atomic emission spectroscopy (ICP-AES) and transmission electron microscopy coupled with an energy dispersive X-ray analysis (TEM-EDXA), which also helped to elucidate the mechanism of the specific staining. Intracellular selective staining may be correlated with the microenvironment of the nucleolus, which is consistent with experiments conducted at different phases of the cell cycle. These results prove that N1 is a very attractive phosphorescent staining reagent for visualizing the nucleolus of living cells. (C) 2013 Elsevier Ltd. All rights reserved.
语种	英语
出版者	ELSEVIER SCI LTD
内容类型	期刊论文
源URL	[http://dspace.xmu.edu.cn/handle/2288/88026]
专题	海洋环境－已发表论文
推荐引用方式 GB/T 7714	Chen, Min,Lei, Zhen,Feng, Wei,et al. A phosphorescent silver(I)-gold (I) cluster complex that specifically lights up the nucleolus of living cells with FLIM imaging[J]. http://dx.doi.org/10.1016/j.biomaterials.2013.02.032,2013.
APA	Chen, Min.,Lei, Zhen.,Feng, Wei.,Li, Chunyan.,Wang, Quan-Ming.,...&李春园.(2013).A phosphorescent silver(I)-gold (I) cluster complex that specifically lights up the nucleolus of living cells with FLIM imaging.http://dx.doi.org/10.1016/j.biomaterials.2013.02.032.
MLA	Chen, Min,et al."A phosphorescent silver(I)-gold (I) cluster complex that specifically lights up the nucleolus of living cells with FLIM imaging".http://dx.doi.org/10.1016/j.biomaterials.2013.02.032 (2013).