Maximizing Antibody Production in Suspension-Cultured Mammalian Cells by the Customized Transient Gene Expression Method

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	Maximizing Antibody Production in Suspension-Cultured Mammalian Cells by the Customized Transient Gene Expression Method
	You, Min ; Liu, Yanning ; Chen, Yingwei ; Guo, Jiyuan ; Wu, Jun ; Fu, Yajuan ; Shen, Rong ; Qi, Rui ; Luo, Wenxin ; Xia, Ningshao ; Luo WX(罗文新) ; Xia NS(夏宁邵)
刊名	http://dx.doi.org/10.1271/bbb.120968
	2013
关键词	HAMSTER OVARY CELLS RECOMBINANT PROTEIN-PRODUCTION HIGH-LEVEL TRANSFECTION OPTIMIZATION TEMPERATURE DESIGN VIRUS LINES
英文摘要	National Natural Science Foundation of China [81161120415]; Significant New Drug Creation, Special Science and Technology Major Projects [2011ZX09102-009-12]; International Cooperation and Exchange Project [2012DFH30020]; Policy Guidance Project of the Ministry Science and Technology [2011FU125Z04]; Due to the great diversity in protein expression productivity, a customized transient gene expression (TGE) method was used in the present study to optimize transient expression of three antibodies. Several factors, including host cells, temperature, valproic acid (VPA) treatment, various vectors, and additives were optimized independently and then combined to form a customized TGE protocol for each antibody. In the event, the optimized TGE conditions for three antibodies were different from each other. Compared with the TGE in CO-S cells by pCDNA3.1 expression vector, the expression productivities of 8C11 cAb, 37 hAb, and 10F7 cAb showed 16-fold, 293-fold, and 19-fold increases respectively by the customized TGE method. For 8C11 cAb, coexpressing L-chain and H-chain on different plasmids led to higher yields. The customized TGE method is an alternative approach that can greatly improve the expression productivity of a variety of recombinant proteins.
语种	英语
出版者	TAYLOR & FRANCIS LTD
内容类型	期刊论文
源URL	[http://dspace.xmu.edu.cn/handle/2288/88014]
专题	海洋环境－已发表论文
推荐引用方式 GB/T 7714	You, Min,Liu, Yanning,Chen, Yingwei,et al. Maximizing Antibody Production in Suspension-Cultured Mammalian Cells by the Customized Transient Gene Expression Method[J]. http://dx.doi.org/10.1271/bbb.120968,2013.
APA	You, Min.,Liu, Yanning.,Chen, Yingwei.,Guo, Jiyuan.,Wu, Jun.,...&夏宁邵.(2013).Maximizing Antibody Production in Suspension-Cultured Mammalian Cells by the Customized Transient Gene Expression Method.http://dx.doi.org/10.1271/bbb.120968.
MLA	You, Min,et al."Maximizing Antibody Production in Suspension-Cultured Mammalian Cells by the Customized Transient Gene Expression Method".http://dx.doi.org/10.1271/bbb.120968 (2013).