The Effect of an Adding Histidine on Biological Activity and Stability of Pc-pis from Pseudosciaena crocea

CORC > 厦门大学 > 海洋环境－已发表论文

	The Effect of an Adding Histidine on Biological Activity and Stability of Pc-pis from Pseudosciaena crocea
	Mao, Yong ; Niu, Sufang ; Xu, Xin ; Wang, Jun ; Su, Yongquan ; Wu, Yang ; Zhong, Shengping ; Wang J(王军)
刊名	http://dx.doi.org/10.1371/journal.pone.0083268
	2013
关键词	ANTIMICROBIAL PEPTIDE CRYPTOCARYON-IRRITANS HYDROPHOBIC INTERACTIONS EPINEPHELUS-COIOIDES AEROMONAS-HYDROPHILA MEMBRANE INTERFACES MAST-CELLS PISCIDIN ANALOGS FISH
英文摘要	State 863 Project of China [2012AA10A403]; National Natural Science Foundation of China [41176115, 31372504]; Pc-pis is a novel piscidin-like antimicrobial polypeptide that was identified in Pseudosciaena crocea. Although active against most bacteria tested, Pc-pis was inactive against Aeromonas hydrophila and Pseudomonas aeruginosa. The Pc-pis analogue Pc-pis-His was designed by adding a histidine residue at the carboxyl terminal. Pc-pis-His demonstrated a more broad-spectrum and stronger antimicrobial activity against a representative set of microorganisms and more potent antiparasitic activity against Cryptocaryon irritans trophonts than Pc-pis. The stability assay revealed that Pc-pis-His was active against Staphylococcus aureus not only in acidic (pH 5.5-7.3) and relatively low concentration monovalent cation (0-160 mM NaCl) environments but also in alkaline (pH 7.5-9.5), divalent cation (1.25-160 mM MgCl2 and 1.25-40 mM CaCl2) and high concentration monovalent cation (320-2560 mM NaCl) environments, which indicates that the added histidine residue conferred better salt-, acid- and alkali-tolerance to Pc-pis-His. Pc-pis-His also possessed the desired heat-tolerance, which was reflected by the antimicrobial activity of the peptide after being boiled for 10-60 minutes. Hemolytic activity analysis revealed that Pc-pis-His at concentrations up to 6 mu M exhibited no hemolysis against human erythrocytes, with 6 mu M being a concentration that is highly active against most of the microorganisms tested, although the hemolytic activity of Pc-pis-His was enhanced compared to Pc-pis. These results provide a unique, reasonable basis for designing novel piscidins with potent, broad-spectrum and stable antimicrobial activity and new insight into the future development of piscidins as potential therapeutic agents against microbial and external protozoan parasite infections.
语种	英语
出版者	PUBLIC LIBRARY SCIENCE
内容类型	期刊论文
源URL	[http://dspace.xmu.edu.cn/handle/2288/87907]
专题	海洋环境－已发表论文
推荐引用方式 GB/T 7714	Mao, Yong,Niu, Sufang,Xu, Xin,et al. The Effect of an Adding Histidine on Biological Activity and Stability of Pc-pis from Pseudosciaena crocea[J]. http://dx.doi.org/10.1371/journal.pone.0083268,2013.
APA	Mao, Yong.,Niu, Sufang.,Xu, Xin.,Wang, Jun.,Su, Yongquan.,...&王军.(2013).The Effect of an Adding Histidine on Biological Activity and Stability of Pc-pis from Pseudosciaena crocea.http://dx.doi.org/10.1371/journal.pone.0083268.
MLA	Mao, Yong,et al."The Effect of an Adding Histidine on Biological Activity and Stability of Pc-pis from Pseudosciaena crocea".http://dx.doi.org/10.1371/journal.pone.0083268 (2013).