An uncultivated crenarchaeota contains functional bacteriochlorophyll a synthase | |
Meng, Jun ; Wang, Fengping ; Wang, Feng ; Zheng, Yanping ; Peng, Xiaotong ; Zhou, Huaiyang ; Xiao, Xiang ; Xiao X(肖翔) | |
刊名 | http://dx.doi.org/10.1038/ismej.2008.85
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2009-01 | |
关键词 | LATERAL GENE-TRANSFER DEEP-SEA SEDIMENT ESCHERICHIA-COLI METAGENOMIC LIBRARY GENOME SEQUENCE ARCHAEA BACTERIA COMMUNITIES DIVERSITY EVOLUTION |
英文摘要 | NSFC [40625016, 40532011]; China Ocean Mineral Resources RD Association [COMRA DYLY0202-01]; A fosmid clone 37F10 containing an archaeal 16S rRNA gene was screened out from a metagenomic library of Pearl River sediment, southern China. Sequence analysis of the 35 kb inserted fragment of 37F10 found that it contains a single 16S rRNA gene belonging to Miscellaneous Crenarchaeotal Group (MCG) and 36 open reading frames (ORFs). One ORF (orf11) encodes putative bacteriochlorophyll a synthase (bchG) gene. Bacteriochlorophyll a synthase gene has never been reported in a member of the domain Archaea, in accordance with the fact that no (bacterio)-chlorophyll has ever been detected in any cultivated archaea. The putative archaeal bchG (named as ar-bchG) was cloned and heterologously expressed in Escherichia coli. The protein was found to be capable of synthesizing bacteriochlorophyll a by esterification of bacteriochlorophyllide a with phytyl diphosphate or geranylgeranyl diphosphate. Furthermore, phylogenetic analysis clearly indicates that the ar-bchG diverges before the bacterial bchGs. Our results for the first time demonstrate that a key and functional enzyme for bacteriochlorophyll a biosynthesis does exist in Archaea. |
语种 | 英语 |
出版者 | ISME J |
内容类型 | 期刊论文 |
源URL | [http://dspace.xmu.edu.cn/handle/2288/87868] ![]() |
专题 | 海洋环境-已发表论文 |
推荐引用方式 GB/T 7714 | Meng, Jun,Wang, Fengping,Wang, Feng,et al. An uncultivated crenarchaeota contains functional bacteriochlorophyll a synthase[J]. http://dx.doi.org/10.1038/ismej.2008.85,2009. |
APA | Meng, Jun.,Wang, Fengping.,Wang, Feng.,Zheng, Yanping.,Peng, Xiaotong.,...&肖翔.(2009).An uncultivated crenarchaeota contains functional bacteriochlorophyll a synthase.http://dx.doi.org/10.1038/ismej.2008.85. |
MLA | Meng, Jun,et al."An uncultivated crenarchaeota contains functional bacteriochlorophyll a synthase".http://dx.doi.org/10.1038/ismej.2008.85 (2009). |
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