An uncultivated crenarchaeota contains functional bacteriochlorophyll a synthase

CORC > 厦门大学 > 海洋环境－已发表论文

	An uncultivated crenarchaeota contains functional bacteriochlorophyll a synthase
	Meng, Jun ; Wang, Fengping ; Wang, Feng ; Zheng, Yanping ; Peng, Xiaotong ; Zhou, Huaiyang ; Xiao, Xiang ; Xiao X(肖翔)
刊名	http://dx.doi.org/10.1038/ismej.2008.85
	2009-01
关键词	LATERAL GENE-TRANSFER DEEP-SEA SEDIMENT ESCHERICHIA-COLI METAGENOMIC LIBRARY GENOME SEQUENCE ARCHAEA BACTERIA COMMUNITIES DIVERSITY EVOLUTION
英文摘要	NSFC [40625016, 40532011]; China Ocean Mineral Resources RD Association [COMRA DYLY0202-01]; A fosmid clone 37F10 containing an archaeal 16S rRNA gene was screened out from a metagenomic library of Pearl River sediment, southern China. Sequence analysis of the 35 kb inserted fragment of 37F10 found that it contains a single 16S rRNA gene belonging to Miscellaneous Crenarchaeotal Group (MCG) and 36 open reading frames (ORFs). One ORF (orf11) encodes putative bacteriochlorophyll a synthase (bchG) gene. Bacteriochlorophyll a synthase gene has never been reported in a member of the domain Archaea, in accordance with the fact that no (bacterio)-chlorophyll has ever been detected in any cultivated archaea. The putative archaeal bchG (named as ar-bchG) was cloned and heterologously expressed in Escherichia coli. The protein was found to be capable of synthesizing bacteriochlorophyll a by esterification of bacteriochlorophyllide a with phytyl diphosphate or geranylgeranyl diphosphate. Furthermore, phylogenetic analysis clearly indicates that the ar-bchG diverges before the bacterial bchGs. Our results for the first time demonstrate that a key and functional enzyme for bacteriochlorophyll a biosynthesis does exist in Archaea.
语种	英语
出版者	ISME J
内容类型	期刊论文
源URL	[http://dspace.xmu.edu.cn/handle/2288/87868]
专题	海洋环境－已发表论文
推荐引用方式 GB/T 7714	Meng, Jun,Wang, Fengping,Wang, Feng,et al. An uncultivated crenarchaeota contains functional bacteriochlorophyll a synthase[J]. http://dx.doi.org/10.1038/ismej.2008.85,2009.
APA	Meng, Jun.,Wang, Fengping.,Wang, Feng.,Zheng, Yanping.,Peng, Xiaotong.,...&肖翔.(2009).An uncultivated crenarchaeota contains functional bacteriochlorophyll a synthase.http://dx.doi.org/10.1038/ismej.2008.85.
MLA	Meng, Jun,et al."An uncultivated crenarchaeota contains functional bacteriochlorophyll a synthase".http://dx.doi.org/10.1038/ismej.2008.85 (2009).