Modulation and Interaction of Immune-Associated Parameters with Antioxidant in the Immunocytes of Crab Scylla paramamosain Challenged with Lipopolysaccharides

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	Modulation and Interaction of Immune-Associated Parameters with Antioxidant in the Immunocytes of Crab Scylla paramamosain Challenged with Lipopolysaccharides
	Gopalakrishnan, Singaram ; Chen, Fang-Yi ; Thilagam, Harikrishnan ; Qiao, Kun ; Xu, Wan-Fang ; Wang, Ke-Jian ; Wang KJ(王克坚) ; Chen FY(陈芳奕)
刊名	http://dx.doi.org/10.1155/2011/824962
	2011
关键词	PROPHENOLOXIDASE-ACTIVATING SYSTEM SHRIMP LITOPENAEUS-VANNAMEI PENAEUS-MONODON IN-VITRO MYTILUS-GALLOPROVINCIALIS ANTIMICROBIAL PEPTIDES SUPEROXIDE-DISMUTASE SICYONIA-INGENTIS REACTIVE OXYGEN INNATE IMMUNITY
英文摘要	National High Technology Research and Development Program of China (863 Program) [2007AA091406]; program for Changjiang Scholars and Innovative Research Team in University (PCSIRT) [IRT0941]; Minjiang Scholar Program; Invertebrates are dependent on cellular and humoral immune defences against microbial infection. Scylla paramamosain is an important commercial species, but the fundamental knowledge on its immune defense related to the antioxidant and immune-associated reactions is still lacking. The study was to differentiate the responses of immune-associated parameters of haemolymph components in S. paramamosain when challenged with bacterial lipopolysaccharides (LPSs). The immunostimulating effects of LPS in crab by triggering various immune parameters (phagocytosis, lysozyme, antibacterial activity, phenoloxidase, and the generation of superoxide and nitric oxide) were investigated. Results showed that the generation of free radicals, phenoloxidase, lysozyme and antibacterial activities was significantly increased through the exposure periods. Conversely, total hemocyte count and lysosomal membrane stability decreased significantly as the exposure period extended to 96 h. The relationship between the antioxidant enzymes and immune reactions due to LPS was highly significant. In addition, ROS production was positively correlated with antioxidant showing immediate response of antioxidant defense to the oxyradicals generated. Overall, the study indicated that nonspecific immune components in hemocytes of crab showed active response to the LPS stimulation, and their responses suggested that many immune-associated parameters could be modulated and interrelated with the influence of antioxidants in crustaceans.
语种	英语
出版者	EVID-BASED COMPL ALT
内容类型	期刊论文
源URL	[http://dspace.xmu.edu.cn/handle/2288/87840]
专题	海洋环境－已发表论文
推荐引用方式 GB/T 7714	Gopalakrishnan, Singaram,Chen, Fang-Yi,Thilagam, Harikrishnan,et al. Modulation and Interaction of Immune-Associated Parameters with Antioxidant in the Immunocytes of Crab Scylla paramamosain Challenged with Lipopolysaccharides[J]. http://dx.doi.org/10.1155/2011/824962,2011.
APA	Gopalakrishnan, Singaram.,Chen, Fang-Yi.,Thilagam, Harikrishnan.,Qiao, Kun.,Xu, Wan-Fang.,...&陈芳奕.(2011).Modulation and Interaction of Immune-Associated Parameters with Antioxidant in the Immunocytes of Crab Scylla paramamosain Challenged with Lipopolysaccharides.http://dx.doi.org/10.1155/2011/824962.
MLA	Gopalakrishnan, Singaram,et al."Modulation and Interaction of Immune-Associated Parameters with Antioxidant in the Immunocytes of Crab Scylla paramamosain Challenged with Lipopolysaccharides".http://dx.doi.org/10.1155/2011/824962 (2011).