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Thermal resistance in sea cucumbers (Apostichopus japonicus) with differing thermal history: The role of Hsp70
Meng, Xian-liang ; Ji, Ting-ting ; Dong, Yun-wei ; Wang, Qing-lin ; Dong, Shuang-lin ; Dong YW(董云伟)
2009
关键词Sea cucumber Thermal resistance Heat shock protein 70 Temperature acclimation
英文摘要Thermal history can affect organisms' thermal resistance, however, effects of thermal history on thermal resistance and roles of heat shock protein 70 (Hsp70) in sea cucumber Apostichopus japonicus are not well understood. In the present study, the lethal thermal limits and expressions of Hsp70 of sea cucumbers that were acclimated at different temperatures (22 degrees C or 12 degrees C) for 30 days were studied. The upper lethal temperatures (ULTs(50)) for sea cucumbers acclimated at 22 and 12 degrees C were 31.8 degrees C (31.5-32.1 degrees C) and 30.9 degrees C (30.6-31.3 degrees C), respectively. In heat shock experiments, sea cucumbers acclimated at different temperatures (22 degrees C or 12 degrees C) were exposed to several high temperatures (25, 27, 29, 31 and 32 degrees C) for 2 h, followed by 2 h recovery at 17 degrees C and the levels of hsp70 were measured using semi-quantitative RT-PCR and western blotting. The maximum induction temperatures for sea cucumbers in 22 degrees C and 12 degrees C acclimation treatments were 31 degrees C and 29 degrees C, respectively. These results showed that the temperature acclimation could affect sea cucumbers' upper thermal limit, which was related to the changed expression pattern of Hsp70 in the sea cucumber. In practice, sea cucumbers that overwinter in southern China could acquire higher thermal resistance than those reared in the north. (c). 2009 Elsevier B.V. All rights reserved.
语种英语
内容类型期刊论文
源URL[http://dspace.xmu.edu.cn/handle/2288/60682]  
专题海洋环境-已发表论文
推荐引用方式
GB/T 7714
Meng, Xian-liang,Ji, Ting-ting,Dong, Yun-wei,et al. Thermal resistance in sea cucumbers (Apostichopus japonicus) with differing thermal history: The role of Hsp70[J],2009.
APA Meng, Xian-liang,Ji, Ting-ting,Dong, Yun-wei,Wang, Qing-lin,Dong, Shuang-lin,&董云伟.(2009).Thermal resistance in sea cucumbers (Apostichopus japonicus) with differing thermal history: The role of Hsp70..
MLA Meng, Xian-liang,et al."Thermal resistance in sea cucumbers (Apostichopus japonicus) with differing thermal history: The role of Hsp70".(2009).
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