Isolation and identification of the immune-relevant ribosomal protein L10 (RPL10/QM-like gene) from the large yellow croaker Pseudosciaena crocea (Pisces: Sciaenidae)

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	Isolation and identification of the immune-relevant ribosomal protein L10 (RPL10/QM-like gene) from the large yellow croaker Pseudosciaena crocea (Pisces: Sciaenidae)
	Chen, X. ; Su, Y. Q. ; Wang, J. ; Liu, M. ; Niu, S. F. ; Zhong, S. P. ; Qiu, F. ; Su YQ(苏永全)
刊名	http://dx.doi.org/10.4238/2012.October.15.7
	2012
关键词	MICROCELL HYBRID-CELLS WILMS-TUMOR SUPPRESSOR MESSENGER-RNA LEVEL MOLECULAR CHARACTERIZATION ATLANTIC SALMON QM EXPRESSION CHALLENGE CLONING TISSUE
英文摘要	Special Fund for Agro-Scientific Research in the Public Interest [200903029]; Natural Science Research Program of Universities in Jiangsu Province, China [12KJD240001]; "Famous Abroad Teacher" Program of Jiangsu, China [(2011)212]; In order to investigate the immune role of ribosomal protein L10 (RPL10/QM-like gene) in marine fish, we challenged the large yellow croaker Pseudosciaena (= Larimichthys) crocea, the most important marine fish culture species in China, by injection with a mixture of the bacteria Vibrio harveyi and V. parahaemolyticus (3: 1 in volume). Microarray analysis and real-time PCR were performed 24 and 48 h post-challenge to isolate and identify the QM-like gene from the gill P. crocea (designated PcQM). The expression level of the PcQM gene did not changed significantly at 24 h post-challenge, but was significantly downregulated at 48 h post-challenge, suggesting that the gene had an immune-modulatory effect in P. crocea. Full-length PcQM cDNA and genomic sequences were obtained by rapid amplification of cDNA ends (RACE)-PCR. The sequence of the PcQM gene clustered together with those of other QM-like genes from other aquatic organisms, indicating that the QM-like gene is highly conserved in teleosts.
语种	英语
内容类型	期刊论文
源URL	[http://dspace.xmu.edu.cn/handle/2288/60656]
专题	海洋环境－已发表论文
推荐引用方式 GB/T 7714	Chen, X.,Su, Y. Q.,Wang, J.,et al. Isolation and identification of the immune-relevant ribosomal protein L10 (RPL10/QM-like gene) from the large yellow croaker Pseudosciaena crocea (Pisces: Sciaenidae)[J]. http://dx.doi.org/10.4238/2012.October.15.7,2012.
APA	Chen, X..,Su, Y. Q..,Wang, J..,Liu, M..,Niu, S. F..,...&苏永全.(2012).Isolation and identification of the immune-relevant ribosomal protein L10 (RPL10/QM-like gene) from the large yellow croaker Pseudosciaena crocea (Pisces: Sciaenidae).http://dx.doi.org/10.4238/2012.October.15.7.
MLA	Chen, X.,et al."Isolation and identification of the immune-relevant ribosomal protein L10 (RPL10/QM-like gene) from the large yellow croaker Pseudosciaena crocea (Pisces: Sciaenidae)".http://dx.doi.org/10.4238/2012.October.15.7 (2012).